Netrin-1在胎儿生长受限及重度子痫前期胎盘中的表达

目的:通过检测Netrin-I在正常足月妊娠、重度子痫前期、胎儿生长受限及重度子痫前期合并胎儿生长受限胎盘中的定位及表达,探讨Netrin-1与妊娠过程中胎盘形成及胎儿生长的关系。方法:分别采用免疫组织化学染色法、实时荧光定量逆转录聚合酶链反应(Real-time PCR)及免疫印迹法(Western blot)检测24例正常足月妊娠、18例重度子痫前期、15例胎儿生长受限及18例重度子痫前期合并胎儿生长受限胎盘中Netrin-1蛋白定位、含量及mRNA的表达水平。结果:Netrin-1定位于细胞滋养细胞及合体滋养细胞的胞浆。real-time PCR及免疫印迹法结果显示,与正常足月胎盘相比,重度子痫前期胎盘Netrin-1mRNA及蛋白水平稍有下降,但无统计学意义(P>0.05),但在胎儿生长受限组及重度子痫前期合并胎儿生长受限组中Netrin-1表达水平显著降低(P<0.01)。结论:孕期滋养细胞是Netrin-1的重要来源之一。Netrin-1参与了妊娠期胎盘形成的调控,可能在胎儿生长过程中发挥重要作用。

Expression of Netrin - 1 in placenta of fetal growth restriction and severe preeclampsia

Objective: To explore the relationship between Netrin-1 and placentation and fetal growth during pregnancy by detecting the expression of Netrin-1 in normal full-term pregnant women,pregnant women complicated by fetal growth restriction(FGR),pregnant women complicated by severe preeclampsia(PE) with and without FGR.Methods: Immunohistochemical staining,real-time PCR,and Western blot were used to detect the location and content of Netrin-1 protein,and expression of Netrin-1 mRNA in 24 normal full term pregnant women,18 pregnant women with severe PE,15 patients with fetal growth restriction(FGR),a nd 18 patients with severe PE combined with FGR.Results: Netrin-1 located in cytoplasm of cytotrophoblast cells and trophoblast cells;Netrin-1 mRNA and protein decreased slightly in placenta of severe PE group(P > 0.05),but the expression level of Netrin-1 in FGR group and FGR combined with severe PE group decreased significantly(P < 0.01).Conclusion: Netrin-1 maybe mainly derived from trophoblast cells during pregnancy.Netrin-1 is involved in the regulation of placentation and may play an important role in fetal growth.