甲状腺癌蛋白质差异表达的观察

目的 由于甲状腺结节的高发率和鉴别良恶性的困难，所以需要寻找一种比较特异的分子标志。本研究的目的就是利用蛋白组学方法，建立人甲状腺癌和癌旁正常组织的双向凝胶蛋白图谱，寻找差异表达的蛋白质。方法 双向凝胶电泳分离人甲状腺癌及其周围正常组织的总蛋白质，通过图形软件分析比较，找出差异表达的蛋白质，利用质谱对差异表达的蛋白质进行鉴定。结果 建立双向凝胶图谱，平均蛋白质点数约为1000个左右。发现只在肿瘤表达的点有263个，表达上调2倍及以上的点有134个。在对差异点进行的质谱鉴定中发现了热休克蛋白27在肿瘤中表达下调。结论 建立了人甲状腺乳头状癌和癌旁正常组织的双向电泳图谱，并鉴定了一些与肿瘤相关的蛋白质。

Analysis of Different Protein Expressions in Thyroid Cancer

To study the different protein expressions in thyroid cancer and the normal tissue adjacent to the tumor in order to find out a useful diagnostic marker of thyroid cancer. Methods The total proteins of human papillary thyroid cancer and paired normal tumor-adjacent tissue were separated by two-dimensional electrophoresis. The differential expressed proteins were analyzed by using PDQuest 7.2 software and identified by using mass spectrometry and database searching. Results There were about 1000 points in each gel, and the points number only existed in tumor gel were 263, 134 points being two folds or more up-expressions. Heat shock 27 was the most interesting differential expression protein and was clown expression in papillary thyroid cancer. Conclusion Two dimensional patterns of human papillary thyroid cancer and paired normal tumor-adjacent tissue have been established and certain differential proteins associated with thyroid cancer are identified.