| HCMV—IgM捕获ELISA法的建立及其应用 |
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| 引用本文： | 胡野,单小云. HCMV—IgM捕获ELISA法的建立及其应用[J]. 中国误诊学杂志, 2002, 2(7): 993-995 |
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| 作者姓名： | 胡野 单小云 |
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| 作者单位： | 1. 浙江省金华职业技术学院医学院,浙江,金华,321111
2. 浙江省金华市中心医院检验科,321111 |
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| 摘 要： | 目的 建立检测人巨细胞病毒 (HCMV)抗原特异性 Ig M的酶联捕获技术 (Capturing- EL ISA)。方法 应用 Capturing- EL ISA法检测 12 8份巨细胞病毒感染者血清中抗 HCMV- Ig M,并与常用的间接 EL ISA法进行比较。结果 抗原浓度、抗 HCMV抗体酶结合物浓度对所测得的标本 OD值都有不同程度的影响 ,用最适的实验条件建立的 Cap-turing- EL ISA法特异性及敏感性均高于间接 EL ISA法 ,且不受 RF因子的影响。结论 Capturing- EL ISA敏感性高、特异性强 ,且简便、快速 ,重复性好 ,具有实际应用价值
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| 关 键 词： | 巨细胞病毒属/免疫学 IgM 巨细胞病毒感染/诊断 酶联免疫吸附测定 |
| 文章编号： | 1009-6647(2002)07-0993-03 |
| 修稿时间： | 2002-03-10 |
Establishment of Capturing-ELISA for the Detection of Anti-HCMV-IgM and Its Application |
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| HU Ye,SHAN Xiao-yun. Establishment of Capturing-ELISA for the Detection of Anti-HCMV-IgM and Its Application[J]. Chinese Journal of Misdiagnostics, 2002, 2(7): 993-995 |
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| Authors: | HU Ye SHAN Xiao-yun |
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| Abstract: | Objective To establish capturing-E LISA for the detection o f anti-HCMV-IgM in serum.Method Capturing ELISA and indirect ELISA methods we re used and compared to detect-HCMV-IgMs in 128 patients with HCMV infection. R esults The results showed that the concentration of antigen and enzyme conjugat ed anti-HCMV antibody could affect the OD values.The spe cificity and sensitivi ty of capturing-ELISA with optimal condition were shown to be significantly hig her than that of indirect ELISA,and its results were not affected by RF factor. Conclusions Such a specific,sensitive,convenient and reliable method mi ght be applicable. |
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| Keywords: | Cytomegalovirus/immunology IgM Cytomeg alovirus infections/diagnosis Enzyme-linked immunosorbent assay |
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