胰腺癌中MAT1蛋白的表达与其临床病理特征的关系

目的：研究MAT1蛋白在胰腺癌中的表达及其与胰腺癌多项临床病理特征的关系。 方法： 使用免疫组化首先分析了70例外科手术切除的胰腺癌标本、10例胰腺良性肿瘤、14例慢性胰腺炎和10例尸解来源正常胰腺组织标本中的MAT1蛋白表达。然后比较MAT1蛋白的表达与胰腺癌患者多项临床病理特征的关系，包括患者年龄、性别、癌灶部位、癌灶大小、组织学类型、分化程度和TNM分期。 结果： MAT1蛋白主要表达于胰腺癌癌细胞，也可见于成纤维细胞，棕黄色染色局限于细胞浆和核膜。75.7％（53/70）的胰腺癌组织中可见MAT1蛋白的表达，而正常对照标本中则未表达或仅微弱表达MAT1。MAT1在4种组织中的表达程度有显著差异（P<0.01）。其中MAT1在胰腺癌中的表达显著高于其它3种组织。进一步分析发现，MAT1蛋白表达与胰腺癌患者的TNM分期呈显著相关（P<0.05），但与其它临床病理特征无关。 结论： 在胰腺癌中表达明显上调的MAT1蛋白可能与胰腺癌的发生相关，并且MAT1蛋白是评估胰腺癌患者预后的一个有用的肿瘤标志物。

Relationship between MAT1 expression and clinicopathological features of pancreatic cancers

AIM: To investigate the expression of MAT1 protein in pancreatic cancers and the relationship between MAT1 and clinicopathological features of pancreatic cancers. METHODS: 94 surgical specimens, including 70 pancreatic cancers, 10 pancreatic benign tumors, 14 chronic pancreatitis and 10 autopsy normal pancreas tissues, were analyzed immunohistochemically, and then MAT1 expression and clinicopathological features were compared. RESULTS: MAT1 was expressed mainly in the cancer cells,and also in the fibroblasts, where it was localized within the cytoplasm and nuclear envelope. MAT1 expression was found in 75.7% (53/70) of the cancers, but not detected or weakly expressed in control tissues. There was a significant difference in expression of MAT1 among the above four tissues (P<0.01). Furthermore, MAT1 expression showed a significant correlation with TNM stage (P<0.05), but no correlation with other clinicopathological features. CONCLUSION: The MAT1 protein that is up-regulated in the pancreatic cancer may involve in pancreatic carcinogenesis, and MAT1 in primary lesion may be an useful marker for evaluating prognosis in patients with pancreatic cancer.