组织因子在人翼状胬肉中的表达

目的研究分析组织因子（TF）在人翼状胬肉和正常结膜组织中的表达。方法手术切除翼状胬肉组织13例和正常球结膜组织5例。福尔马林固定,石蜡包埋后的组织进行免疫组化分析TF的表达,并用免疫组织化学双标记染色法分析TF和α-平滑肌肌动蛋白（α-SMA）或表皮生长因子受体（EGFR）在翼状胬肉中的表达。结果所有翼状胬肉组织中均检测到TF的表达。TF位于基底细胞、基底上层细胞、表层上皮细胞胞浆中。翼状胬肉TF阳性上皮细胞数量明显高于正常结膜中TF阳性上皮细胞（P〈0.001）。α-SMA阳性或阴性的翼状胬肉上皮细胞中均检测到TF的表达。翼状胬肉上皮细胞中检测到EGFR的表达,并且与TF表达在同一位置。结论TF人翼状胬肉中表达上调,在翼状胬肉发生、发展中起着潜在作用,TF的表达可能是通过依赖EMT和非依赖EMT的途径实现的。

Expression levels of tissue factor in human pterygium

Objective To Analyze the expression of TF in pterygial and normal conjunctival tissues of humans.Methods Thirteen pterygia and five normal bulbar conjunctivas,surgically removed,were used in this study.Formalin-fixed,paraffin-embedded tissues were submitted for immunohistochemical analysis with anti-TF antibody.Double staining immunohistochemistry was performed to assess TF and alpha-smooth muscle actin(α-SMA) or epidermal growth factor receptor(EGFR) expression in the pterygia.Results Immunoreactivity for TF was detected in all pterygial tissues examined.TF immunoreactivity was localized in the cytoplasm of basal,suprabasal,and superficial epithelial cells.The number of TF-immunopositive cells in pterygial epithelial cells was significantly higher than in normal conjunctival epithelial cells(P<0.001).TF immunoreactivity was detected in α-SMA-positive or-negative pterygial epithelial cells.EGFR immunoreactivity was detected in pterygial epithelium,which was colocalized with TF.Conclusion These results suggest that TF plays a potential role in the pathogenesis and development of a pterygium,and that TF expression might be involved through EMT-dependent and-independent pathways.