| 重组人乳铁蛋白的遗传毒性研究 |
| |
| 引用本文： | 刘珊,冯晓莲,李晨汐,耿桂英,刘海波,支媛,徐海滨. 重组人乳铁蛋白的遗传毒性研究[J]. 癌变．畸变．突变, 2011, 23(5): 384-387. DOI: 10.3969/j.issn.1004-616x.2011.05.015 |
| |
| 作者姓名： | 刘珊 冯晓莲 李晨汐 耿桂英 刘海波 支媛 徐海滨 |
| |
| 作者单位： | 中国疾病预防控制中心营养与食品安全所,北京,100021 |
| |
| 基金项目： | 国家高技术研究发展计划(863计划)项目(2007AA100506); 转基因生物新品种培育科技重大专项(2008ZX08011-005) |
| |
| 摘 要： | 目的：评价重组人乳铁蛋白的遗传毒性。方法：以重组人乳铁蛋白为受试物,按每皿62、185、556、1667、5000μg5个剂量进行Ames试验,按1.88、3.75和7.50g/kg进行小鼠骨髓嗜多染红细胞（PCE）微核试验和精子畸形试验,并设立对照组。结果：重组人乳铁蛋白各剂量组回变菌落数均未超过溶剂对照菌落数的2倍,亦无剂量-反应关系;各剂量组PCE百分比均未少于阴性对照组的20%,微核发生率与阴性对照组比较差异均无统计学意义（P均〉0.05）;各剂量组小鼠精子畸形发生率均显著低于阴性对照组（P〈0.05）。结论：在本实验条件下,重组人乳铁蛋白未见遗传毒性。
|
| 关 键 词： | 遗传毒性 重组蛋白 人乳铁蛋白 |
Genetic toxicity of recombinant human lactoferrin |
| |
| LIU Shan,FENG Xiao-lian,LI Chen-xi,GENG Gui-ying,LIU Hai-bo,ZHI Yuan,XU Hai-bin. Genetic toxicity of recombinant human lactoferrin[J]. Carcinogenesis,Teratogenesis and Mutagenesis, 2011, 23(5): 384-387. DOI: 10.3969/j.issn.1004-616x.2011.05.015 |
| |
| Authors: | LIU Shan FENG Xiao-lian LI Chen-xi GENG Gui-ying LIU Hai-bo ZHI Yuan XU Hai-bin |
| |
| Affiliation: | LIU Shan,FENG Xiao-lian,LI Chen-xi,GENG Gui-ying,LIU Hai-bo,ZHI Yuan,XU Hai-bin~* (Institute for Nutrition and Food Safety,China Center for Disease Control and Prevention,Beijing 100021,China) |
| |
| Abstract: | OBJECTIVE:To evaluate genetic toxicity of recombinant human lactoferrin(rhLf) expressed in milk from genetically modified cow.METHODS:In Ames test,TA97,TA98,TA100,and TA102 strains were treated with 62,185,556,1 667 and 5 000μg recombinant human lactoferrin(rhLf) per plate.In mice bone marrow cell micronucleus test and mice sperm abnormality test,1.88,3.75 and 7.50 g/kg rhLf groups and control groups were set.RESULTS:Back mutation colonies of rhLf groups did not exceed more than twice that of the control in Ames test and there was no dose-response relationship.In mice bone marrow cell micronucleus test,no significant difference was found in PCE/RBC and micronucleus rate between rhLf-treated groups and negative control group.In mice sperm abnormality test,sperm abnormality rate of rhLf-treated groups was lower than that of negative control.CONCLUSION:No genetic toxicity was observed in recombinant human lactoferrin in this study. |
| |
| Keywords: | genetic toxicity recombinant protein human lactoferrin |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
