| 免疫球蛋白测定方法的探讨及其临床应用 |
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| 引用本文： | 夏林,李荷华,董新善. 免疫球蛋白测定方法的探讨及其临床应用[J]. 武汉大学学报(医学版), 1994, 0(2) |
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| 作者姓名： | 夏林 李荷华 董新善 |
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| 作者单位： | Department of Clinical Labortory，Second Affiliated Hosipital，Hubei Medical University，Wuhan 430071，China |
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| 摘 要： | 用三种方法测定血清免疫球蛋白（Ｉｇ）含量，并进行了彼此间差异的显著性和精密度的比较，认为三种方法测定血清Ｉｇ的含量无显著性差异（Ｐ＞０．０５），彼此间相关性较好（ｒ＝０．９７７）。而用免疫比浊法（ＩＴＭ）测定血清Ｉｇ的精密度远高于单向免疫扩散法（ＲＩＤ）和火箭免疫电泳法（ＥＩＡ）。用免疫比浊法测定ＩｇＧ、ＩｇＡ、ＩｇＭ的正常参考值（Ｘ±ｓ）分别为１２．１３±１．５１ｇ／Ｌ、２．０４±０．５５ｇ／Ｌ、１．１４±０．２７ｇ／Ｌ。慢性活动性肝炎ＩｇＧ、ＩｇＡ水平１９．８８±４．９６ｇ／Ｌ３．３３±０．６８／Ｌ与正常人比较差异有高度显著性（Ｐ＜０．０１）；ＩｇＭ水平１．２６±０．２４ｇ／Ｌ与正常人比较无显著性差异（Ｐ＞０．０５）．同时用免疫比浊法测定ＩｇＧ、ＩｇＡ、ＩｇＭ有较好的回收率。
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| 关 键 词： | 免疫测定／方法；免疫球蛋白类／血液；化学．临床／方法；对比研究 |
Evaluation and Clinic Application a Assay Methods for the Detection of Immunoglobin |
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| Xia Lin,Li Hekua, Dong Xinshan. Evaluation and Clinic Application a Assay Methods for the Detection of Immunoglobin[J]. Medical Journal of Wuhan University, 1994, 0(2) |
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| Authors: | Xia Lin Li Hekua Dong Xinshan |
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| Abstract: | Three methods were used to detect serum immnoglobin and their precision and statistical significance compared. We thought there was not significant difference between these methods(P>0. 05)and there was a high correlation(r=0.97). Precition of immunoturbidimetry(ITM) is far higher than that of radical immunodiffusion(RID) and rocket eletroimmunoassay (EIA). Normal reference values of ITM detecting IgG, IgA, IgM, were 12.13±1.51 g/L2.04±0.55 g/L1. 14±0. 27 g/L. Respectively IgG, IgA, level values of chronic active hepatitis were 19. 88±4. 96 g/L, and 3. 33±0. 68 g/L, and compared with the normal there was significant difference(P<0.01). IgM Value was 1.26±0.24 g/L, compared with normal there being not significant difference(P>0. 05) in comparison with the normal.Moreover, there were good retrieval. |
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| Keywords: | immunoassay/MT Immunoglobulins/BL chemistry clinical/MT compartive study |
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