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Complement depletion affects demyelination and inflammation in experimental allergic neuritis |
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| Authors: | Francine J Vriesendorp Robyn E Flynn Miguel A Pappolla Carol L Koski |
| Institution: | aDepartment of Neurology, University of Texas Health Science Center, 6431 Fannin, Room 7.044, Houston, TX 77030, USA;bDepartment of Pathology and Laboratory Science, University of Texas Health Science Center, 6431 Fannin, Houston, TX 77030, USA;cDepartment of Neurology, University of Maryland School of Medicine, Baltimore, MD, USA |
| Abstract: | The effect of systemic complement depletion by cobra venom factor (CVF) on experimental allergic neuritis (BAN) was studied in rats immunized with variable amounts of bovine peripheral nerve myelin. Low-dose myelin EAN rats treated with CVF i.p. (n = 10) had lower clinical scores (0.3 ± 0.7 vs. 1.1 ± 1.1), less demyelination (0.4 ± 0.8 vs. 1.9 ± 1.1) and inflammation (0.6 ± 1.2 vs. 2 ± 1) than EAN animals treated with i.p. saline (n = 10). Endoneurial infiltrates had fewer EDI-positive (phagocytic) macrophages (if0.4 ± 0.5 vs.> 1.6 ± 1.1) and CD11bc-positive (expressing iC3b receptor or CR3) cells (1 ± 0.8 vs. 2.5 ± 0.8) (mean ± S.D.) detected by immunocytochemistry. This effect was partially abrogated by immunizing animals with a higher dose of myelin. Our studies suggest that complement may play a role in the recruitment of macrophages into the endoneurium and in opsonizing myelin for phagocytosis. |
| Keywords: | Experimental allergic neuritis Complement Macrophage Demyelination |
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