| IL-21重组载体的构建及其诱导T细胞活化增殖的研究 |
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| 引用本文： | 刘琳琳.IL-21重组载体的构建及其诱导T细胞活化增殖的研究[J].中华临床医师杂志(电子版),2014(17):69-71. |
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| 作者姓名： | 刘琳琳 |
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| 作者单位： | 山东医学高等专科学校医学检验系,山东临沂276002 |
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| 摘 要： | 目的构建小鼠IL-21基因真核重组表达载体,为IL-21基因治疗肿瘤奠定基础。方法分离BALB/c小鼠脾细胞,提取总RNA,经RT-PCR扩增IL-21基因片段,构建重组质粒pcDNA3.1(-)-IL-21,分组免疫小鼠,检测其脾细胞膜表面CD分子。结果重组质粒测序结果与GenBank中序列一致,大小为490 bp。核酸疫苗免疫组小鼠脾细胞表面CD分子表达量明显高于PBS和pcDNA3.1(-)空质粒组。结论成功的构建了重组质粒pcDNA3.1(-)-IL-21,该核酸疫苗免疫小鼠后,可有效地诱导其体内T细胞的活化增殖。
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| 关 键 词： | T淋巴细胞 白细胞介素21 基因克隆 |
Activation and proliferation of T lymphocyte with cloning of IL-21 gene |
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| Liu Linlin.Activation and proliferation of T lymphocyte with cloning of IL-21 gene[J].Chinese Journal of Clinicians(Electronic Version),2014(17):69-71. |
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| Authors: | Liu Linlin |
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| Institution: | Liu Linlin( Department of Pathogeny Biology and Immunology, Shandong Medical College, Linyi 2 76002, China) |
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| Abstract: | Objective In order to establish the foundation of the tumor therapy, we construct the recombinant interleukin 21 eukaryotic expression plasmid.Methods The IL-21 gene was amplified from lymphocytes from spleens of the BALB/c mice. After 10 days from the mice were immunized, we assayed the CD of lymphocytes.Results The sequence of IL-21 gene amplified was in accordance with that in GenBank database, which was 490 bp. The CD levels of group with pcDNA3.1（-）-IL-21 were higher than the PBS and pcDNA3.1（-） groups.Conclusion The recombinant interleukin 21 eukaryotic expression plasmid was constructed successfully. The vaccine can induce the effective activation and proliferation of T lymphocyte. |
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| Keywords: | T-lymphocytes Interleukin 21 Gene clone |
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