广西菲牛蛭中一种纤溶酶的分离纯化

目的从广西菲牛蛭消化液中分离纯化出一种纤溶酶,并研究其生化性质。方法应用有机溶剂沉淀、DEAESepharoseCL-6B离子交换柱和SephadexG-75凝胶柱过滤的方法分离纯化出广西菲牛蛭纤溶酶,用纤维蛋白平板法测定其纤溶活力,SDS-聚丙烯酰胺凝胶电泳测定纤溶酶相对分子质量(Mr)。结果从广西菲牛蛭中分离出一种单一组分的纤溶酶,其Mr约为42×103,在普通纤维蛋白平板和加热平板上都能溶解纤维蛋白,收率为0.43%。结论此方法成功地从广西菲牛蛭中分离出一种纤溶酶,其纤溶机理是直接溶解纤维蛋白。

The purification of a fibrinolysin from Hirudinaria Manillensis of Guangxi

Purpose To purify a fibrinolysin from the digestive juice of Guangxi Hirudinaria Manillensis and to study its biochemical nature.Methods The fibrinolysin from Hirudinaria Manillensis of Guangxi was precipitated with organic solvent and purified by DEAE Sepharose CL-6B ion-exchange chromatography and Sephadex G-75 gel filtration.Its fibrinolytic activity was determined by fibrin plate and its molecular weight was calculated by SDS-PAGE.Results A kind of purified fibrinolysin was obtained from Hirudinaria Manillensis of Guangxi.This protein possessed fibrinolytic activity and its molecular weight was estimated to be 42×103 and its yield was about 0.43%.Conclusion It was a successful method by which a fibrinolysin from Hirudinaria Manillensis of Guangxi could be purified.This fibrinolysin mechanism was directly dissolving fibrin.