阿奇霉素对人肺腺癌多药耐药细胞株A549/ADM的耐药逆转研究

目的研究阿奇霉素（AZM）对人肺腺癌多药耐药细胞株A549／阿霉素（ADM）的耐药逆转作用。方法使用四甲基偶氮唑盐（MTT）法检测不同浓度AZM对细胞株A549／ADM的抑制率，确定其非细胞毒性浓度。用MTT法分别检测ADM、顺铂（DDP）、长春新碱（VCR）、氨甲蝶呤（MTX）对细胞株A549、A549／ADM以及使用AZM处理后的A549／ADM的半数抑制浓度（IC50）。使用流式细胞仪检测A549以及加入AZM前后的A549／ADM内ADM的荧光强度。结果≤10mg／L的AZM对A549／ADM无明显细胞毒作用。A549／ADM对ADM、VCR、DDP、MTX均有不同程度的耐药。使用5mg／LAZM处理后，A549／ADM对上述4种药物的敏感度均有不同程度的提高。AZM的浓度提高至10mg／L时，A549／ADM对上述药物的敏感程度提高更明显。A549／ADM经AZM处理后细胞内ADM荧光强度明显提高。结论AZM可以部分逆转A549／ADM的耐药，其逆转效果与剂量相关。

Reversal effect on human lung adenocarcinoma multidrug resistance cell line A549/ADM by azithromycin

Objective To investigate the reversal effect of azithromycin on human lung adenocarcinoma multidrug resistance cell line A549/adriamycin(ADM).Methods The non-cytotoxic concentration of azithromycin(AZM) to A549/ADM was determined with MTT assay. The IC_ 50 of ADM,DDP,VCR and MTX to A549,A549/ADM and A549/ADM treated with AZM were detected with MTT assay. The fluorescence intensity of ADM in A549 and A549/ADM with or without AZM treated were detected with flow cytometry (FCM). Results No more than 10 mg/L AZM has no obvious cytotoxicity to A549/ADM cell line. A549/ADM to ADM,DDP, VCR, MTX were drug resistance incoordinately than A549. With AZM added(5 mg/L), sensitivities of A549/ADM to ADM, DDP,VCR,MTX all increased incoordinately. The sensitivities of A549/ADM to these drugs were much higher when treated with 10mg/L AZM. The fluorescence intensity of ADM in A549/ADM treated with AZM increased obviously. Conclusion AZM could partially reverse the drug resistance of A549/ADM and the effect of reversal correlates with concentrations.