HPLC法测定颈复康颗粒剂中葛根素含量

目的：是建立可控的颈复康颗粒剂含量测定方法。方法：采用反相高效液相色谱法测定颈复康颗粒剂中葛根素的含量，以十八烷基硅烷键合硅胶为填料，甲醇：水（25：75）为流动相，检测波长为250nm。结果：该方法能理想地分离颈复康颗粒剂中的葛根素，并测定其含量。葛根素平均回收率为102.07%，精密度（RSD）为3.18%(n=5)。结论：所建立的含量测定标准可行，可控。测定结果准确，能有效地反映颈复康颗粒剂的内在质量。

DETERMINATION OF PUERARIN IN JING FU KANG GRANULES BY HPLC

Objective: To establish an accurate and effective method of assay for qualification standardof Jing Fu Kang granules. Method: Used RP-HPLC method to determine the content of puerarin in JingFu Kang Granules. Using ODS as fixed phase, methanol-water (25:75)as the mobile phase, and the detec-tion wavelength was 250nm. Result: This method can seperate puerarin perfectly, thc average recovery ofpuerarin was 102.07%and RSD was3.18% (n=5). Conclusion: This method of assay for qualificationstandard of Jing Fu Kang Granule is reliable and accurate.