| 检测大鼠细小病毒抗体的ELISA、IEA和HI法比较 |
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| 引用本文： | 贺争鸣,卫礼,张然,刘佐民,吴惠英.检测大鼠细小病毒抗体的ELISA、IEA和HI法比较[J].实验动物与比较医学,1993(3). |
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| 作者姓名： | 贺争鸣 卫礼 张然 刘佐民 吴惠英 |
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| 作者单位： | 中国药品生物制品检定所，中国药品生物制品检定所，中国药品生物制品检定所，中国药品生物制品检定所，中国药品生物制品检定所 |
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| 摘 要： | 对检测大鼠细小病毒(RV株)抗体的ELISA、IEA和HI三种方法作了比较。ELISA、IEA和HI法的重复性分别为92.5％、88.2％和68.6％。经对同一批血清检品的检测,ELISA法IEA和检测结果的符合率为90.5％,抗体阳性检出率均为81％,而HI与前两法的符合率均仅为69％,抗体阳性检出率为69％。以上结果表明:ELISA和IEA法在重复性和抗体阳性检出率方面均优于HI(P<0.05)。经ELISA法检测,普通级大鼠中RV抗体阳性率达59％,而在清洁级大鼠中仅为7％。
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| 关 键 词： | 酶联免疫吸附试验 免疫酶试验 血凝抑制试验 大鼠 细小病毒 |
Comparison among ELISA, IEA and HI for Detection of Rat Parvovirus Antibody |
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| He Zheng-ming,Wei Li,Zhang Ran,Liu Zuo-min,Wu Hui-ying National Institute for the Control of Pharmaceutical and Biological Products,Beijing.Comparison among ELISA, IEA and HI for Detection of Rat Parvovirus Antibody[J].Laboratory Animal and Comparative Medicine,1993(3). |
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| Authors: | He Zheng-ming Wei Li Zhang Ran Liu Zuo-min Wu Hui-ying National Institute for the Control of Pharmaceutical and Biological Products Beijing |
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| Institution: | He Zheng-ming,Wei Li,Zhang Ran,Liu Zuo-min,Wu Hui-ying National Institute for the Control of Pharmaceutical and Biological Products,Beijing 100050 |
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| Abstract: | Comparison among ELISA, IEA and HI was made in detecting the antibody torat Parvovirus. The reproducibilities of ELISA, IEA and HI were 92.5%. 88.2%and 68.6% respectively. When the same sera were investigated, the consistencies ofthe results between ELISA and IEA, HI and ELISA/IEA were 90.5% and 69% re-spectively,and antibody-positive rates were 81% by both ELISA and IEA, but only69% by HI. The reproducibilities of the seromonitoring results and the positiverates of antibody by ELISA and IEA were higher than by HI (P<0.05), RVantibody was found positive by ELISA in 59% of the conventional rat samples whilein clean rat, the positive rate was only 7%. |
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| Keywords: | ELISA IEA HI Rat Parvovirus |
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