宫颈癌巨噬细胞浸润和VEGFR-3表达与淋巴结转移的关系

目的 :探讨宫颈癌组织巨噬细胞浸润与VEGFR-3阳性脉管密度的相关性及其与淋巴结转移的关系。 方法 :采用免疫组化SP法检测59例宫颈癌石蜡标本中CD68及VEGFR-3的蛋白表达,并应用计算机辅助图像分析系统对脉管的密度行定量分析。 结果: 淋巴结转移组巨噬细胞数和VEGFR-3阳性脉管密度明显高于无淋巴结转移组(P<0.05、P<0.01)。随临床分期或病理分级增高,巨噬细胞数和VEGFR-3阳性脉管密度均有增加的趋势,但无统计学差异(P>0.05)。巨噬细胞数和VEGFR-3阳性脉管密度成正相关(r=0.318,P=0.014)。 结论: 宫颈癌中浸润的巨噬细胞通过促进淋巴管生成与宫颈癌的淋巴结转移密切相关。

The Relationship between Macrophages Infiltration and VEGFR-3 Positive Vessel Density in Uterine Cervical Cancers

Objective: To investigate the correlation between macrophages and VEGFR-3 positive vessel density in uterine cervical cancers and their relationship with lymphatic metastasis. Methods: The CD68 and VEGFR-3 were identified by immunohistochemically in the 59 parafin specimens respectively. The density of VEGFR-3 positive vessel was assessed quantitatively by Computer-assisted video analysis system. Results: The mean macrophage counts and VEGFR-3 positive vessel density were significantly higher in the lymph node metastasis group than in the non-metastasis group (P<0.05,P<0.01). The mean macrophage counts and VEGFR-3 positive vessel density did not correlated with tumor stage and clinical stage. A positive correlation was found between the mean macrophage counts and VEGFR-3 positive vessel density in tumors (P=0.014). Conclusions: Macrophages infiltration triggers lymphatic angiogenesis in cervical cancers, which may be closely related to lymph node metastasis.