舒普深、哌拉西林钠的胶束电动毛细管色谱分析

 目的 建立精密、快速简便地测定舒普深 (内含头孢哌酮和舒巴坦)及注射用哌拉西林含量的方法。方法 采用胶束电动毛细管色谱法（MECC），测定运行液采用pH=7.5的0. 02 mol·L^-1磷酸盐缓冲液，其中含十二烷基硫酸钠(SDS)11 g·L^-1, 正丁醇22 mL·L^-1,正庚烷4 mL·L^-1。分离电压18 kV(运行电流85～95 μA)。结果 3种成分在10～20 min内得到完全分离，选择苯巴比妥作内标，各组分浓度在0.1～2 g·L^-1范围内浓度对内标峰面积之比呈良好线性关系。可准确测定3种成分的含量，批内RSD均小于1%(n=8)，3种成分的加样回收率均在94.92%～105.3%之间，RSD均小于3.4%(n=8)。结论 用MECC同时测定头孢哌酮、舒巴坦、哌拉西林钠的含量，方便易行，结果准确可靠。

Determination of sulperazon and piperacillin by micellar electrokinet ic capillary chromatography

OBJECTIVE To establish a method for the determination of the content of cefoperazone and sulbactam in sulperazon and piperacillin injection.METHOD The complete separation of the components was achieved by micellar electrokinetic capillary chromatography (MECC) with 0.02 mol·L^-1 pH7.5 running phosphate buffer containing SDS 11 g·L^-1,1-butanol 22 mL·L^-1 and heptane 4 mL·L^-1.The constant voltage was 18 kV ( current of 85～95 μA).RESULTS The migration times of the individual components were between10 and 20 minutes with phenobarbital as the internal standard.Good linearity over the range of 0.1～2 g·L^-1 was obtained.The RSD of intra-run was less than 1%(n=8) and the recoveries were 94.92%～105.3% with RSD less than 3.4%(n=8).CONCLUSION This method was accurate,simple and rapid. The quantity of each components may be determined using this method.