Loss of p53 in benzene-induced thymic lymphomas in p53+/- mice: evidence of chromosomal recombination |
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Authors: | Boley S E Anderson E E French J E Donehower L A Walker D B Recio L |
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Affiliation: | Chemical Industry Institute of Toxicology, Research Triangle Park, North Carolina 27709, USA. |
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Abstract: | The purpose of this study was to examine the role of chromosomal recombination in mediating p53 loss in benzene-induced thymic lymphomas in C57BL/6-Trp53 haploinsufficient (N5) mice (p53+/- mice). We characterized loss of heterozygosity (LOH) on chromosome 11 using seven microsatellite markers in 27 benzene-induced and 6 spontaneous thymic lymphomas. Eleven patterns of LOH were found between the induced and spontaneous tumors, with only one pattern being in common between the tumor groups. Nearly 90% (24 of 27) of benzene-induced tumors exhibited loss of the functional p53 allele locus, and 83% (20 of 24) of these tumors retained two copies of the disrupted p53 allele. The results indicate that benzene induces a high frequency of LOH on chromosome 11 in p53+/- mice, likely mediated by aberrant chromosomal recombination. |
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