丙戊酸钠的血药浓度监测及合理用药分析

目的建立人血清中丙戊酸钠浓度测定方法。方法以二氯甲烷为提取溶剂，。一溴苯乙酮为衍生试剂，环己烷羧酸为内标，采用高效液相色谱法测定，色谱柱：HypersilODS-C18（4．6mm×250mm，5μm）不锈钢色谱柱，流动相：甲醇一水（75：25），检测波长：248nm。流速：1．0mL·min^-1。结果丙戊酸钠浓度在25--150μg·mL^-1。范围内苯甲酰甲基丙戊酸和苯甲酰甲基环己烷羧酸的峰面积比与丙戊酸钠浓度呈现良好线性关（R2=0．9953），日内和日间RSD〈4．19％。结论该方法快速、简便、准确。适合于牯床血药浓度监测。

Determination of valproic acid sodium blood drug concentration and analysis of the rationality

OBJECTIVE To establish an analysis method for the determination of sodium valprate in the serum. METHODS Determination was performed with HPLC with methanol：water （75：25） as the mobile phase ω-bromine acetophenone as the deriving agent and cyclohexanecarboxylic acid as the internal standard . The analytical column was Hypersil ODS-C18（4.6mm×250mm,5μm）stainless steel column, velocity： 1.0 mL·min^-1, detected at 248nm. RESULTS The linear rang of sodium valproic was 25-150μg·mL^-1 within R2 = 0.9953 . Both the relative standard deviation of within day and between day was less than 5.1%. CONCLUSIONS This method is rapid, simple , accurate and suitable for clinical drug concentration monitoring.