聚合酶链反应扩增STY3671基因鉴别伤寒与甲型副伤寒沙门菌

目的 评价伤寒沙门菌基因STY3671用于特异性检测该血清型沙门菌的可能性。 方法 根据伤寒沙门菌与甲型副伤寒沙门菌外膜蛋白质组比较得到的伤寒沙门菌特异性蛋白点编码基因STY3671的序列设计引物,提取伤寒沙门菌、甲型副伤寒沙门菌以及其他非伤寒沙门菌血清型菌株的基因组DNA进行PCR检测, 对引物特异性和灵敏性进行分析。 结果 检测的184株伤寒沙门菌均得到807 bp长度片段,部分菌株扩增产物测序结果与伤寒沙门菌标准株CT-18一致。146株甲型副伤寒沙门菌以及其余163株其他血清型沙门菌均未扩增出该片段。 结论 STY3671基因能够作为特异性检测伤寒沙门菌的靶标,能够区别于甲型副伤寒沙门菌及其他常见非伤寒沙门菌血清型,在发热患者的伤寒沙门菌感染诊断、尤其使用血液标本检测中具有良好的潜在价值。

Identification of Salmonella typhi and Salmonella paratyphi A by PCR amplification of gene STY3671

Objective To evaluate the feasibility of using gene STY3671 in detection of Samonella typhi.MethodsProteomics comparison between outer membrane proteins of S.typhi and S.paratyphi A was conducted,one of the specific dots was identified,and primers were designed according to its coding sequence,PCR test was performed by using epidemic strains of Salmonella,then the sensitivity and specificity of the assay were evaluated.Results A 807 bp PCR fragment was successfully amplified from all 184 strains of S.typhi,random sequencing of the fragments showed coincidence with gene STY3671 in strain CT-18.No fragment was amplified from any S.paratyphi A and other Salmonella strains.Conclusion STY3671 is a specific target to distinguish S.typhi from S.paratyohi A and other Salmonella,this assay has high sensitivity and specificity and might be used in diagnosis of typhoid fever.