云南省大理州发热患者血液中检测斑点热群立克次体

目的 调查云南省大理州不明原因发热患者是否存在斑点热。 方法 用巢式-聚合酶链反应法扩增患者血液中的立克次体属groEL基因,并进行核酸序列鉴定和分析。 结果 20例患者血液中检出1例立克次体核酸阳性(检出率5%),其序列分析与GenBank中Rickcttsia rickcttsii Iowa、Rickcttsia peacockii Rustic、Rickcttsia sibirica 等斑点热群立克次体株的同源性最高(为99%)。 结论 首次证实云南大理地区存在斑点热患者, 医疗部门应加强对斑点热的诊断与鉴别。

The detection of spotted fever group Rickettsia DNA from fever patients in Dali,Yunnan

Objective To identify the pathogen of patients with fever with unknown origin and determine whether they were infected with spotted fever group Rickettsia.Methods Nested polymerase chain reaction(nPCR) was conducted to amplify groEL gene of rickettsia from the blood samples of the patients,the nucleotide sequencing and identification were carried out.Results Rickettsial DNA was detected in 1 of the 20 patients(5%).The groEL gene of the strain shared high homology(99%) with Rickcttsia rickcttsii Iowa,Rickcttsia peacockii Rustic and Rickcttsia sibirica.Conclusion It was the first time to diagnose the patient with spotted fever in Dali,Yunnan province.It is necessary to enhance the differential diagnosis of spotted fever in local medical institutions.