腹泻样本中创伤弧菌的检测分析

目的 了解浙江省部分地区腹泻患者创伤弧菌的感染情况。 方法 对2010年6-10月采集的标本,经3%氯化钠碱性蛋白胨水于37 ℃增菌后,划线纤维二糖多粘菌素E平板于40 ℃培养18~24 h,挑取可疑菌落进行分子生物学鉴定。 结果 在144份腹泻患者粪便样本中检测分离到2株创伤弧菌,阳性率为1.39%(2/144);均为生物Ⅰ型,其基因型为vcgC/16S rRNA B型。药敏结果显示分离的创伤弧菌对氨基糖苷类抗生素耐药如阿米卡星、庆大霉素。 结论 在监测的腹泻样本中分离到具有较强致病能力的创伤弧菌,且氨基糖苷类抗生素耐药,应加强对海水产品和腹泻样本中创伤弧菌的检测及耐药分析,预防创伤弧菌的感染暴发。

Detection of Vibrio vulnificus in stool samples from diarrhea patients in Zhejiang province

Objective To understand the infection status of Vibrio vulnificus in diarrhea patients in selected areas in Zhejiang.Methods The alkaline peptone water with 3% NaCl was used to enrich the target pathogen in stool samples at 37 ℃ for 6 to 9 hours,a loop of enrichment broth was streaked to cellobiose polymyxin E plate and cultivated at 40 ℃ for 18 to 24 hours,and the suspicious clones were isolated for identification and genotyping.Results Two Vibrio vulnificus strains were isolated from 144 stool samples(1.39%),they belonged to biotypeⅠand vcgC/16S rRNA B genotype.The drug susceptibility test indicated that the 2 Vibrio vulnificus strains were resistant to aminoglycoside antibiotics,which included amikacin and gentamicin.Conclusion The strains of Vibrio vulnificus with strong virulence and drug resistance were isolated from the stool samples of diarrhea patients.It is necessary to strengthen the detection of Vibrio vulnificus in sea foods and stool samples from diarrhea patients and the analysis of its drug resistance to prevent the outbreak of Vibrio vulnificus infection.