应用3种方法检测鼠疫恢复期患者血清中F1抗体水平

目的 了解鼠疫恢复期患者血清F1抗体水平的维持情况及间接血凝、酶联免疫、胶体金免疫层析技术在鼠疫F1抗体检测中的应用。 方法 应用间接血凝试验(IHA)、双抗原夹心酶联免疫吸附试验(DAgs-ELISA)和胶体金免疫层析试验(GⅠCA)3种方法,对比检测25例鼠疫恢复期患者血清中F1抗体。 结果 25份血清样品F1抗体IHA阳性19份,DAgs-ELISA阳性21份,GⅠCA阳性18份。F1抗体滴度:IHA 15例为1∶80~1∶320;ELISA 10例为1∶640~1∶1280。 结论 大部分鼠疫恢复期患者血清能检出F1抗体,3种F1抗体检测方法均为敏感、特异的检测技术。

Detection of F1 antibody levels in convalescent sera from plague patients with three assays

Objective To understand the duration of F1 antibodies in the sera from plague patients in convalescent period and the effectiveness of three detecting assays of indirect hemagglutination test(IHA),double-antigen sandwich enzyme-linked immunosorbent assay(DAgs-ELISA)and colloidal gold immunochromatography(GICA).MethodsTwenty five serum samples from plague patients in convalescent period were detected with IHA,DAgs-ELISA and GICA,respectively.Results Among the 25 serum samples,19,21 and 18 were detected to be positive in IHA test,DAgs-ELISA and GICA,respectively.F1 antibody titers were 1∶80-1∶320 in 15 positive samples in IHA test,and 1∶640-1∶1280 in 10 positive samples in DAgs-ELISA.Conclusion F1 antibodies could be detected in convalescent sera in most plague patients by conducting three assays above mentioned,indicating they are sensitive and specific in plague detection.