氯化锂对人骨肉瘤细胞U2-OS增殖、凋亡及Fas、Caspase-3 mRNA表达的影响

目的:研究氯化锂(LiCl)对人骨肉瘤细胞U2-OS增殖及凋亡的影响,并探讨其可能的机制。方法:以40、80、150mmol/L的LiCl作用于U2-OS细胞24、48和72h后,采用MTT法检测细胞增殖抑制率;作用48h后,流式细胞术法检测细胞凋亡率,并分析细胞周期;RT-PCR法检测凋亡相关基因Fas、Caspase-3mRNA的表达。结果:随LiCl作用剂量的增加,U2-OS细胞增殖抑制率增加,细胞凋亡率和Fas、Caspase-3mRNA表达量亦增加(F=61157.480,70.828和418.072,P<0.001);细胞周期分析显示细胞被阻滞于S期(P<0.001)。结论:LiCl可能通过促进凋亡基因Fas、Caspase-3的表达,抑制U2-OS细胞增殖并诱导凋亡。

Effect of lithium chloride on proliferation,apoptosis and mRNA expressions of Fas and Caspase-3 in human osteosarcoma U2-OS cell

Aim:To investigate the effect of lithium chloride(LiCl) on proliferation and apoptosis of human osteosarcoma cell U2-OS,and probe the possible mechanism.Methods:U2-OS cells were cultured with different concentrations of LiCl(40,80,and 150 mmol/L) for 24,48,and 72 h,respectively,then the growth inhibitory effects were detected with MTT.Apoptosis and cell cycle was observed by flow cytometry,and Fas and Caspase-3 mRNA expression,by RT-PCR for cells cultured for 48 h.Results:LiCl could inhibit the growth of U2-OS cells,induce apoptosis(F = 61 157.480,P < 0.001) in a dose-dependent manner.The cell cycle analysis revealed that the cells were arrested in S checkpoint.LiCl could increase the expression of Fas and Caspase-3 mRNA obviously(F = 70.828,418.072,P < 0.001).Conclusion:LiCl could inhibit the proliferation and induce apoptosis of U2-OS cells through increasing the expression of Fas and Caspase-3.