不同化疗药物对人肝癌耐药细胞BEL-7404/ADM端粒酶活性抑制的研究

目的:观察几种常用化疗药物对人肝癌耐药细胞BEL-7404/ADM端粒酶活性的影响.方法:利用端粒重复扩增实验(TRAP)结合非变性聚丙烯酰胺凝胶电泳银染法,测定BEL-7404/ADM耐药细胞经过多种化疗药物顺铂(DDP)、阿霉素(ADM)、丝裂霉素(MMC)、长春新碱(VCR)]不同浓度和时间作用后端粒酶活性的变化.结果:当各种药物高浓度处理细胞24 h后再去除药物,DDP对端粒酶的活性完全抑制,MMC有部分抑制,ADM和VCR无抑制作用;低浓度药物处理细胞3 d,其结果同高浓度相似.结论:高浓度DDP和MMCC在短时间内或低浓度长时间作用对人肝癌耐药细胞BEL-7404/ADM端粒酶活性有显著抑制作用,抑制作用可能与药物的作用浓度和时间有关.

THE EFFECTS OF DIFFERENT DRUGS ON TELOMERASE ACTIVITY OF LIVER CANCER BEL-7404/ADM CELLS

Objective:To investigate the inhibition of telomerase activity by several chemotherapeutic drugs regularly used in human liver cancer BEL-7404/ADM cells.Methods:By using telomerase repeated amplification protocol(TRAP) combined with PAGE silver staining,we detected the change of telomerase activity of human liver cancer cell line BEL-7404/ADM by DDP,ADM,MMC,and VCR at different concentration(c) and different time periods.Result:In high(c),the telomerase activity of cells was completely inhibited by cisplatin and partly inhibited by mitomycin C when the drug was removed after cells were recultured for 24 hours at high(c) drug treatment.However,doxorubicin and vincristine had no effect.At low(c),3d after drug treatment the effects were similar.Conclusion:Cisplatin and mitomycin C of high(c) treated for a short time period or low(c) of a long time period can inhibit telomerase activity of human liver cancer BEL-7404/ADM cell,which maybe correlates with its dose and time period of the drug.