MMP-2、MMP-9、Cathepsin-D在淋巴结阴性乳腺癌中的表达及其与预后的关系

目的研究MMP-2、MMP-9(matrixmetalloproteinase,基质金属蛋白酶)、Cathepsin-D(组织蛋白酶D)在淋巴结阴性乳腺癌中的表达与预后的关系。方法用免疫组织化学ABC法检测270例淋巴结阴性乳腺癌患者肿瘤组织中MMP-2、MMP-9、Cathepsin-D的表达。结果肿瘤组织中MMP-2、MMP-9、Cathepsin-D的阳性率分别为56·7%(153/270)、59·6%(161/270)、55·6%(150/270);单因素分析MMP-2阳性、MMP-9阳性及两者共表达患者有较低的无复发生存率,多因素分析发现MMP-2和MMP-9共表达能独立预测患者的无复发生存率(RR=2·487;P<0·05)。结论在淋巴结阴性乳腺癌患者中MMP-2和MMP-9的阳性表达患者的预后较差。

Prognostic significance of MMP-2、MMP-9 、Cathepsin-D expression in lymph node-negative breast carcinoma

Objective To evaluate the prognostic significance of MMP-2 , MMP-9 and Cathepsin-D expression in lymph node-negative human breast carcinoma. Methods Specimens from the primary breast tumors of 270 axillary lymph node negative breast cancer patients were studied for MMP-2 , MMP-9 and Cathepsin-D protein expression by immunohistochemical staining. Results The positive expression of MMP-2, MMP-9 and Cathepsin-D was 56.7%(153/270),59.6%(161/270) and 55.6%(150/270), respectively. In univariate analysis, MMP-2, MMP-9 and the co-expression of MMP-2 and MMP-9 were significantly associated with tumor free survival. In multivariate analysis, co-expression of MMP-2 and MMP-9 was unfavorablely related with tumor free survival(RR=2.487; P<0.05). ConclusionMMP-2 and MMP-9 are unfavorable prognostic factors in node-negative breast cancer patients.