联合甲磺酸伊马替尼和ABT-737对胃肠间质瘤细胞凋亡的影响

目的 观察联合甲磺酸伊马替尼(IM)和ABT-737对胃肠间质瘤细胞凋亡的影响.方法 培养胃肠间质瘤细胞株GIST-882；噻唑蓝(MTT)比色法分析不同浓度ABT-737和不同处理时间对GIST-882细胞生长的影响；分析联合应用不同浓度IM和ABT-737对GIST882细胞生长的影响.Western blot法分析IM处理对bcl-2蛋白的影响；并分析联合应用IM和ABT-737对Caspase-3 蛋白表达的影响.结果 10 μmol/L的ABT-737作用细胞24、48、72 h后,细胞的生存率分别是70％、55％、38％；20 μmol/L的ABT-737作用细胞24、48、72 h后,细胞的生存率均小于10％.0.1μmol/L的IM联合0.1μmol/L的ABT-737可将GIST-882细胞的生存率抑制在20％以下,10 μmol/L的IM联合10 μmol/L的ABT-737可将GIST-882细胞的生存率抑制在10％以下.IM对GIST882细胞bcl-2蛋白的表达无明显影响；联合应用IM和ABT-737可以促进GIST-882细胞活化型Caspase-3 蛋白的表达.结论 联合应用ABT-737可以加强IM对GIST细胞的促凋亡作用.

Influence of imatinib combined with ABT-737 on apoptosis of gastrointestinal stromal tumor cells

Objective To observe the effect of imatinib (IM) combined with ABT-737 on apoptosis of gastrointestinal stromal tumor cells.Methods We treated the GIST-882 with ABT-737 alone or ABT737 combined with imatinib.The effect of different concentrations of ABT-737 at different time points on the cell viability was determined by methyl thiazol tetrazolium (MTT).The expression of bcl-2 in GIST882 after IM treatment alone was analyzed by Western blotting.The activation form of Caspase-3 after IM or ABT-737 treatment alone and combination of IM with ABT-737 was analyzed by Western blotting.The effect of combination of IM with ABT-737 on the cell viability was determined by MTT.Results After treatment of GIST-882 cells with ABT-737 alone at a concentration of 10 μmol/L,the 24-,48- and 72-h survival rate of tumor cells was respectively 70％,55％ and 38％.At the concentration of 20 μmol/LABT-737,the 24-,48- and 72-h survival rate was less than 20％.After treatment with IM at a concentration of 0.1 μmol/L combined with ABT-737 at a concentration of 0.1 μmol/L,the survival rate of GIST-882 cells was less than 20％.The combined use of IM with ABT-737 both at the concentration of 10 μmol/L could reduce the survival rate of GIST-882 cells to ＜ 10％.IM treatment alone did not have any effect on the expression of bcl-2.The activation form of Caspase-3 was upregulated by the treatment of combination of IM with ABT-737.Different concentrations of IM combined with ABT-737 could decline the cells viability.Conclusion Combination of ABT-737 with IM could enhance the cell apoptosis.