高容量血液滤过对多脏器功能障碍综合征犬CD14+单核细胞白细胞-DR抗原表达的影响

目的 观察高容量血液滤过(HVHF)对多脏器功能障碍综合征(MODS)犬CD14+单核细胞犬白细胞DR抗原(DLA-DR)表达的影响.方法 通过2次打击建立犬MODS模型,随机分HVHF组和MODS组,HVHF组建模后给予HVHF治疗24h,MODS组不给HVHF治疗.监测CD14+单核细胞DLA-DR表达及主要器官功能指标变化.结果 与实验前(6.52±1.47)比较,MODS组CD14+单核细胞DLA-DR明显降低(P＜0.01).在T5、T7、T8、T9时间点HVHF组CD14+单核细胞DLA-DR水平显著高于MODS组(P＜0.05).HVHF组主要器官功能明显改善.结论 HVHF能改善器官功能,提高CD14+单核细胞DLA-DR的表达,有助于重建机体内稳态.

Effect of high volume hemofiltration on dog leucocyte antigen-DR expression of CD14 + monocytes in dogs with multiple organ dysfunction syndrome

Objective To investigate the effect of high volume hemofiltration (HVHF) on the dog leucocyte antigen-DR (DLA-DR) expression of CD14 + monocytes in dogs with multiple organ dysfunction syndrome (MODS).Methods Dogs were subjected to hemorrhagic shock plus resuscitation and endotoxemia to set up MODS model,and randomly divided into two groups:HVHF group and MODS group.HVHF was performed about 24 h in HVHF group.DLA-DR expression and organ functions were evaluated in blood simultaneously.Results The DLA-DR expression level on CD14 + monocytes in MODS group was significantly lower than the normal level (6.52 ± 1.47,P ＜ 0.01 ).The DLA-DR expression level on CD14 + monocytes in HVHF group was significantly higher than MODS group at T5,T7,T8,T9 (P ＜0.01 ).In HVHF group,the functions of major organs were improved.Conclusion HVHF could improve some organ functions and increase the DLA-DR expression in CD14 + monocytes in dogs with MODS.