骨髓间充质干细胞对大鼠慢性胰腺炎中星状细胞的影响

目的 观察骨髓间充质干细胞(BMSCs)对慢性胰腺炎(CP)中星状细胞(PSC)的活化、增殖和分化的影响.方法 将80只SD大鼠随机分为空白组(20只,注射无菌生理盐水)、模型组[20只,尾静脉注射二氯二丁基酯(DBTC)制作大鼠CP模型]、治疗组(20只,于CP模型制作成功后尾静脉注射体外培养的同种异体GFP-MSCs)、假治疗组(20只,于CP模型制作成功后尾静脉注射等量的无菌生理盐水).取大鼠胰腺检测PSC活化表达的Desmin、胶质纤维酸性蛋白(GFAP)、α-平滑肌肌动蛋白(α-SMA)水平,组织中Ⅰ、Ⅲ胶原含量及白细胞介素(IL)-10、肿瘤坏死因子(TNF)-α、转化生长因子(TGF) -β1表达水平.结果 治疗组PSC表达的Desmin、GFAP、α-SMA,组织中Ⅰ (0.135±0.030) ng/g、Ⅲ胶原含量(0.029±0.008) ng/g,TGF-β1 (0.020±0.006) μg/L浓度均低于假治疗组(P＜0.05),但IL-10 (603.799±89.374) g/ml,TNF-α(507.45±90.13) μg/L均高于假治疗组(P＜0.05),后者与模型组之间的差异无统计学意义(P＞0.05),空白组未见明显异常.结论 BMSCs可以通过对细胞因子的调控减少PSC的活化,抑制其增殖及分化,降低细胞外基质的沉积.

Effects of bone marrow mesenchymal stem cells on pancreatic stellate cells of rats with chronic pancreatitis

Objective To observe the effects of pancreatic stellate cells (PSC) of rats with chronic pancreatitis (CP) after injection of the bone marrow mesenchymal stem cells (BMSCs).Methods The 80 SD rats were randomly divided into four groups:control group (20 rats were injected with sterile saline),model group [20 rats were injected with dibutyltin dichloride (DBT) through the tail vein to make CP model ],treatment group (20 rats were injected with allogeneic GFP-MSCs cultured in vitro when the model of CP was established),and sham treatment group (20 rats were injected with equivalent sterile saline when the model of CP was established).The expression of desmin,glial fibrillary acidic protein (GFAP),α-smooth muscle actin (α-SMA),collagen type Ⅰ and Ⅲ,TGF-β1,IL-10 and TNF-α was detected in the rat pancreas of each group.Results In treatment group,the activity of collegen type Ⅰ (0.135±0.030) ng/g,Ⅲ (0.029±0.008) ng/g,TGF-β1 (0.020±0.006) μg/L in PSC was lower than in sham treatment group ( P ＜ 0.05 ),while that of IL-10 (603.799 ± 89.374) g/ml and TNF-α(507.45 ±90.13) μg/L was higher.All of them had no statistically significant difference between sham treatment group and model group ( P ＞ 0.05 ).No obvious exception was observed in control group.Conclusion By controlling cytokines,BMSCs could reduce the activation,proliferation and differentiation of PSC and lower the deposition of extracellular matrix.