miRNA-21在甲状腺乳头状癌组织中的表达及其意义

目的 检测人甲状腺乳头状癌标本中微小RNA(miRNA) -21的表达,并探讨其意义.方法 采用miRNA芯片技术和TaqMan探针荧光定量聚合酶链反应(PCR)方法,分别检测miRNA-21在3例和20例甲状腺乳头状癌和癌旁组织标本中的表达.结果 应用miRNAs芯片技术,对甲状腺乳头状癌标本进行检测,与癌旁组织比较,甲状腺乳头状癌筛选出的miRNA-21表达上调有统计学意义(P＜0.01)；应用荧光定量PCR技术,检测出甲状腺乳头状癌标本中miRNA-21的平均表达水平较癌旁组织上调(3.97±1.29)倍,差异有统计学意义(P＜0.05).结论 miRNA-21显著高表达可能在甲状腺乳头状癌发生发展过程中起着重要的作用；miRNA芯片技术和TaqMan探针荧光定量PCR方法对minRNA21的检测结果具有一致性.

Expression of miRNA-21 in papillary thyroid carcinoma

Objective To observe the expression of miRNA-21 in papillary thyroid carcinoma (PTC) and adjacent tissues,and to explore the role of miRNA-21 in PTC.Methods The miRNA-21 was measured by micRNA chip technology in 3 cases,and the miRNA-21 was quantified by real-time fluorescence quantitative polymerase chain reaction (PCR) with TaqMan? MicroRNA Assays in 20 cases.Results The expression of miRNA-21 was up-regulated in PTC tissues as compared with than in adjacent non-tumor tissues by micRNA chip technology (P ＜ O.01 ).The mean expression level of miRNA-21 was (3.97 ± 1.29 ) times higher in PTC tissues than that in adjacent non-tumor tissues ( P ＜ 0.05 ).Conclusion The increased expression of miRNA-21 may play an important role in the pathogenesis of PTC.The results from micRNA chip technology and real-time fluorescence quantitative PCR with TaqMan? microRNA Assays in micRNA-21 are consistent.