骨髓间充质干细胞对大鼠慢性胰腺炎炎性因子的影响

目的 观察骨髓间充质干细胞(BMSCs)对大鼠慢性胰腺炎(CP)炎性因子的影响。方法 将80只SD大鼠随机分为空白组(20只,注射无菌生理盐水),模型组[20只,尾静脉注射二丁基二氯化物(DBTC)制作大鼠CP模型],假治疗组(20只,尾静脉注射DBTC制作大鼠CP模型后,输入和BMSCs等量的无菌生理盐水)和治疗组(20只,于CP模型制作成功后尾静脉注射体外培养的同种异体BMSCs)。80 d后采用酶联免疫吸附试验(ELISA)法检测各组大鼠胰腺中白细胞介素(IL)-1、IL-6、IL-8、IL-10、肿瘤坏死因子(TNF)-d、转化生长因子(TGF)-β水平。结果 治疗组中IL-1 (652.326±36.424) ng/L,IL-6(8.347±1.094) ng/L,IL-8( 352.487±69.340) ng/L,rGF-β(0.020±0.006) μg/L,均低于假治疗组与模型组(P＜0.05),IL-10(603.799±89.374) ng/L,TNF-α( 507.450±90.130)μg/L则高于假治疗组和模型组(P＜0.05)。模型组和假治疗组之间IL-1、IL-6、IL-8、IL-10、TNF-α、TGF-β的表达水平差异无统计学意义(P＞0.05)。结论 同种异体BMSCs的输入可以降低CP中促炎因子的水平,提高抗炎因子的浓度,有助于减轻CP的炎症反应,减缓甚至阻止CP的发展。

Effects of bone marrow mesenchymal stem cells on inflammatory factors of rats with chronic pancreatitis

Objective To observe the effects of inflammatory factors in rats with chronic pancreatitis (CP) after injection of the bone marrow mesenchymal stem cells (BMSCs). Methods The 80 SD rats were randomly divided into four groups: control group (20 rats were injected with sterile saline), model group[20 rats were injected with dibutyltin dichloride (DBTC) through tail vein to make CP model],sham treatment group (20 rats were injected with sterile saline in the CP model) and treatment group (20 rats were injected with allogeneic BMSCs cultured in vitro in the CP model). After 80 days, interleukin (IL)-1, IL-6, IL-8, IL-10, tumor necrosis factor (TNF)-α, transforming growth factor (TGF)-β in the rat pancreas of each group were determined. Results IL-1 (652. 326 ± 36. 424) ng/L, IL-6 (8. 347 ± 1. 094) ng/L, IL-8 (352. 487 ± 69. 340) ng/L, TGF-β (0. 020 ± 0. 006) μg/L in treatment group were lower than those in sham treatment group and model group ( P ＜ 0. 05 ), while IL-10 (603. 799 ± 89. 374) ng/L and TGF-α (507. 450 ± 90. 130) μg/L in treatment group were higher than those in sham treatment group and model group ( P ＜ 0. 05). However, there were no statistically significant differences in various inflammatory factors between sham treatment group and model group (P ＞ 0. 05 ). ConclusionAllogeneic BMSCs could reduce the levels of pro-inflammatory cytokines and increase the concentration of anti-inflammatory factors in CP, which is helpful to reduce the inflammation of CP and prevent the development of CP.