| 大骨节病患者与健康人软骨细胞转化生长因子-β mRNA的表达差异 |
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| 引用本文： | 李若飞,高大龙,涂忠民,杨雷刚,董舒,任军龙,解俊杰,赵昕.大骨节病患者与健康人软骨细胞转化生长因子-β mRNA的表达差异[J].实用骨科杂志,2012,18(9):803-806. |
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| 作者姓名： | 李若飞 高大龙 涂忠民 杨雷刚 董舒 任军龙 解俊杰 赵昕 |
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| 作者单位： | 陕西省咸阳市中心医院骨一科,陕西,咸阳,712000 |
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| 摘 要： | 目的测定成人大骨节病（kaschin-beck disease,KBD）患者和正常健康成人关节软骨中转化生长因子-beta（transform growth factor-beta,TGF-β）在mRNA水平表达的差异,为进一步探讨大骨节病的发病机制奠定基础。方法提取成人大骨节病患者和健康人关节软骨细胞总RNA,逆转录得到cDNA;设计针对TGF-β基因的特异引物,通过RT-PCR技术检测该目的基因在mRNA水平表达差异。结果成人大骨节病患者的关节软骨细胞TGF-βmRNA表达低于健康成人。经统计学分析（t=11.1732,P〈0.001）,差异具有统计学意义（P〈0.05）。结论成人大骨节病患者软骨细胞中TGF-β基因的表达低于健康成人,为进一步探讨大骨节病的发病机制和防治奠定了基础。
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| 关 键 词： | 大骨节病 转化生长因子-beta 基因表达 |
Differences of Expression of Transformation Growth Factor Beta mRNA in Articular Cartilage between KBD Patients and Healthy People |
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| Institution: | LI Ruo-fei,GAO Da-long,TU Zhong-min,et al(1st Orthopeadic Department of Xianyang Central Hospital,Xianyang 712000,China) |
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| Abstract: | Objective To determinate the differences of transformation growth factor beta(TGF-β) mRNA expression in articular cartilage between Kaschin-Beck disease(KBD) patients and healthy people.Methods Total RNA was extract from articular cartilage from KBD patients and healthy adults.TGF-βgene expression was determined by RT-PCR.Results Expressions of TGF-β mRNA in articular cartilage cells of KBD patients was lower than that of healthy adult.And there was a statistically significant difference(P<0.05).Conclusion Expression of TGF-β gene in cartilage cells of KBD adult patients was lower than that of healthy adults. |
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| Keywords: | kaschin-beck disease transformation growth factor beta gene expression |
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