碳化硅对成骨细胞增殖和分化的影响

目的研究碳化硅(SiC)的体外生物相容性,为探索SiC作为骨缺损修复材料的可行性奠定基础。方法实验组为实质SiC,对照组为致密羟基磷灰石(HA)。将取自大鼠胎鼠颅骨的成骨细胞分别接种于2组材料表面。分别采用扫描电子显微镜、四甲基偶氮唑盐(MTT)法、碱性磷酸酶(ALP)含量测定技术、流式细胞仪检测细胞周期,观察分析两种材料表面上的细胞的增殖和分化能力。结果扫描电子显微镜显示:原代成骨细胞在两种材料表面伸展良好,细胞在材料表面伸出很多伪足,并且在材料表面可见明显的细胞外基质沉积。两组材料上细胞的MTT值从1、3、5、7 d随观察时间逐渐升高,并且各个时间点实质SiC均高于致密HA,两者之间的差异有统计学意义(P<0.05)。两组材料上细胞的ALP含量从1、3、5、7 d随观察时间逐渐升高;在1、3 d时,致密HA高于实质SiC,在5、7 d时,致密HA低于实质SiC,但是两组之间的差异均无统计学意义(P>0.05)。1、3、5 d两组材料的细胞增值指数(PI)值逐渐升高,高峰出现在第5天,第7天时下降,两组材料在各个时间点的PI均接近,差异无统计学意义(P>0.05)。结论实质SiC具有与致密HA相似的良好的细胞相容性。

Effect of silicon carbide foam on osteoblast proliferation and differentiation

Objective To study the biocompatibility of silicon carbide(SiC) in vitro, and explore the feasibility of SiC as the bone defect repair materials. Methods SiC foam was set as the experimental group, with hydroxyapatite(HA) as the control group. The osteoblasts taken from skulls of fetal rats were seeded on the surface of the two materials. The level of proliferation and differentiation of the osteoblasts were measured with scanning electron microscope, methyl- thiazolyldiphenyl tetrazolium bromide(MTT) assay, and alkaline phosphatase(ALP) assay. Cell cycle was observed by flow cytometry. Results The osteoblasts spread and grew well on both materials, and obvious deposition of extracellular matrix on the material surfaces was observed. The viability of the osteoblasts on SiC material was significantly higher than that on HA material from 1 to 7-days(P < 0.05). ALP value in the osteoblasts on both materials got higher along with the time, but no significant difference was observed between the two materials(P > 0.05) even though the cells on SiC contained more ALP than that on HA. Cell proliferation index(PI) increased with time and peaked at day 5, but no significant difference was observed between 2 materials(P > 0.05). Conclusion It is demonstrated that SiC and HA have similarly good biocompatibility.