| HPLC法测定金钱利胆丸中丹酚酸B的含量 |
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| 引用本文： | 张府君,程永杰,杨兴鑫,李晓妮. HPLC法测定金钱利胆丸中丹酚酸B的含量[J]. 山西中医学院学报, 2012, 13(2): 36-38 |
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| 作者姓名： | 张府君 程永杰 杨兴鑫 李晓妮 |
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| 作者单位： | 山西医科大学药学院,山西太原,030001 |
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| 摘 要： | 目的:建立金钱利胆丸中丹酚酸B含量的测定方法。方法:采用反相高效液相色谱法,Kromasil ODS C18分析柱(5μm,250 mm×4.6 mm),流动相:甲醇-乙腈-甲酸-水(30∶10∶1∶59),检测波长286 nm,流速1.0 mL/min,柱温25℃。结果:丹酚酸B在0.126 8μg/mL～2.536μg/mL范围内线性关系良好(r=0.999 4),平均回收率为96.5%,RSD=1%(n=5)。结论:该法操作简便、准确,适用于金钱利胆丸的质量控制。
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| 关 键 词： | 高效液相色谱法 金钱利胆丸 丹酚酸B |
Determination of Salvianolic Acid B Content in Jinqian Lidan Pill by HPLC |
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| Zhang Fujun , Cheng Yongjie , Yang Xingxin , Li Xiaoni. Determination of Salvianolic Acid B Content in Jinqian Lidan Pill by HPLC[J]. Journal of Shanxi College of Traditional Chinese Medicine, 2012, 13(2): 36-38 |
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| Authors: | Zhang Fujun Cheng Yongjie Yang Xingxin Li Xiaoni |
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| Affiliation: | (College of Pharmacy,Shanxi Medical University,Taiyuan Shanxi 030001) |
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| Abstract: | Objective:To establish a method for determination of salvianolic acid B content in Jinqian Lidan pill.Methods:HPLC was performed on Kromasil ODS C18 column(5 μm,250 mm×4.6 mm)with methanol-acetonitrile-formic acid-water(30∶10∶1∶59)as mobile phase.Detection wavelength was set at 286 nm.Flow rate was at 1.0 mL/min and column temperature was at 25 ℃.Result:The linear range of salvianolic acid B was within the range of 0.126 8 μg/mL ～2.536 μg/mL(r=0.999 4).The average recovery rate was 96.5%,and RSD was 1%(n=5).Conclusion:The method is simple and accurate,and suitable for quality control of Jinqian Lidan pill. |
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| Keywords: | HPLC Jinqian Lidan pill salvianolic acid B |
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