| 新疆产中麻黄不同地理群体遗传关系的RAPD分析 |
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| 引用本文： | 马永红,党荣理,吴霞,刘庆华.新疆产中麻黄不同地理群体遗传关系的RAPD分析[J].中国药学杂志,2003,38(6):414-415. |
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| 作者姓名： | 马永红 党荣理 吴霞 刘庆华 |
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| 作者单位： | 1. 新疆军区卫生防疫大队,新疆,乌鲁木齐,830002
2. 新疆药物研究所,新疆,乌鲁木齐,830000 |
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| 基金项目： | 国家重点科技攻关项目( 2 0 0 0 -K0 1- 0 5- 0 2 ) |
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| 摘 要： | 目的建立新疆3个不同地理群体的中麻黄(Ephdera intermedia)指纹图谱,进行遗传多样性分析。方法通过20个10碱基随机引物对新疆3个不同地理群体的中麻黄进行PCR扩增，并用人工栽培种作为对照，通过计算遗传相似性系数，建立UPGMA聚类图。结果共扩增出153个多态位点，建立了它们的基因组DNA指纹图谱。结论不同地理群体中存在丰富的遗传多样性；相距越远，群体间相似性程度越低；人工栽培种与同一产地野生种具有相似的遗传特性。
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| 关 键 词： | 中麻黄 随机扩增多态DNA 遗传多样性 |
| 文章编号： | 1001-2494(2003)06-0414-02 |
| 收稿时间： | 2002-07-11; |
| 修稿时间： | 2002年7月11日 |
RAPD analysis of genetic relationships in Ephdera intermedia from three local populations in sinkiang |
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| MA Yong hong ,DANG Rong li ,WU Xia ,LIU Qing hua.RAPD analysis of genetic relationships in Ephdera intermedia from three local populations in sinkiang[J].Chinese Pharmaceutical Journal,2003,38(6):414-415. |
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| Authors: | MA Yong hong DANG Rong li WU Xia LIU Qing hua |
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| Institution: | MA Yong hong 1,DANG Rong li 1,WU Xia 2,LIU Qing hua 2 |
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| Abstract: | OBJECTIVE To establish the chromosome DNA fingerprints of Ephdera intermedia from three local populations in Sinkiang and to analyze their genetic diversity.METHODS Twenty different 10 bp oligonucleotide primers of arbitrary sequence were used to amplify with the cultivated specie as control.By calculating the genetic similarity coefficient,UPGMA clustering map was set up.RESULTS A total of 153 polymorphic bands were amplified and the chromosome DNA fingerprints of these local populations were established.CONCLUSION There was rich genetic diversity in different local populations.The far the genetic distances,the lower the comparability degree among populations.And the similar genetic characteristic lies in both the wild specie and the cultivated specie in the same location. |
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| Keywords: | Ephdera intermedia RAPD genetic diversity |
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