重组人骨形成蛋白2诱导颅神经嵴细胞向肾上腺能神经元分化的研究

目的：研究重组人骨形成蛋白2(rhBMP2)诱导小鼠颅神经嵴细胞(CNCC)分化后所产生的神经元类型是否为肾上腺能神经元。方法：用50ng／ml rhBMP2诱导CNCC分化为神经元。提取诱导前后细胞内的总RNA,用反转录聚合酶链反应(RT-PCR)分别检测细胞中酪氨酸羟化酶(TH)和γ-氨基丁酸受体(GABAAR)亚单位的表达情况。结果：诱导前的CNCC中，TH和GABAAR亚单位均无mRNA水平的表达，诱导后二者均有表达，但表达的量均低于脑组织中的表达。结论：rhBMP2诱导CNCC后所分化的神经元为肾上腺能神经元，但在功能上可能弱于正常的肾上腺能神经元。

Recombinant human bone morphogenetic protein 2 induced cranial neural crest cells differentiating into adrenal neurons

Objective:To study whether the differentiated cranial neural crest cells (CNCC) were adrenal neurons after they were induced by recombinant human bone morphogenetic protein 2 (rhBMP 2). Method:CNCC differentiated into neurons after induced by 50 ng/ml rhBMP 2. Total RNA were extracted in rhBMP 2-induced and rhBMP 2-non-induced cells. Expression of Tyrosine hydroxylase (TH) and GABA-alpha receptor (GABAAR) subunit mRNA were detected by RT-PCR in these two groups.Result:]mRNA of TH and GABAAR ( subunit expressed negatively in rhBMP 2-non-induded cells. However, positive signals were found in rhBMP 2-induded cells. The expression of TH and GABAAR subunit were lower in rhBMP 2-induded cells than in brain. mRNA of TH and GABAAR ( subunit expressed negatively in rhBMP 2-non-induded cells. However, positive signals were found in rhBMP 2-induded cells. The expression of TH and GABAAR subunit were lower in rhBMP 2-induded cells than in brain. Conclusion:This suggested that neurons induced by rhBMP 2 were of adrenal phenotype. But the effects of these induced neurons maybe weaker than normal adrenal neurons.