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A colorimetric assay for oxytocinase in pregnancy serum
Authors:M Usategui-Gomez  P Tarbutton  F Yeager
Affiliation:Ames Research Laboratory, Ames Company, Division Miles Laboratories, Inc., Elkhart, Indiana 46514 U.S.A.;Elkhart General Hospital Research Laboratory, Elhhart, Indiana 46514 U.S.A.
Abstract:A simple, rapid colorimetric assay for oxytocinase in pregnancy serum is described. The assay, which utilizes l-cystine-bis-p-nitroanilide as the substrate, is based on the formation of an azo-dye. The assay required a 10-min incubation at 37°, followed by 6 min for color development.
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