H PLC 测定杜仲双降袋泡茶中绿原酸的含量

目的：采用高效液相色谱法（HPLC）建立杜仲双降袋泡茶的质量控制方法。方法采用C18（4．6mm ×150mm ，5μm）色谱柱；流动相为乙腈（A）：0．8％冰醋酸溶液（B）＝6：94；检测波长：327nm ；柱温：35℃；流速：1．0ml／min ；进样量：10μl；结果绿原酸在0．089～1．335μg范围内呈良好的线性关系，线性回归方程为：y ＝3×106x -37523，r ＝1。回收率为101．29％，RSD为0．88％（ n ＝9）。结论该方法简便、快速、准确，能对杜仲双降袋泡茶中绿原酸成分有效地进行质量控制。

HPLC DETERMINATION OF CHLOROGEN IC ACID IN EUCOMMIA BARK TEA IN BAG

Objective To use HPLC to establish a quality control method for the determination of chlorogenic acid in eucommia bark tea .Methods HPLC analysis was performed with C 18 chromatographic column （4 .6 × 150 mm ,5 μm） and a mobile phase of acetonitrile /0 .8% of acetic acid （6/94） .The detection wave length was 327 nm ,the column temperature was 35℃ and the flow rate was 1 .0 ml/min-1 .Results The linear regression equation was y =3 × 106x -37523 （ r = 1） ,linear range was 0 .089~1 .335 μg · mL-1 ,and the average recovery was 101 .29% （ n=9） with RSD being 0 .88 % .Conclusion This method is convenient and accurate ,which can be used for the quality control of chlorogenic acid in eucommia bark tea in bag .