组蛋白乙酰化在二烯丙基二硫抑制CNE2细胞生长增殖中的作用

目的探讨二烯丙基二硫(DADS)在抑制CNE2细胞生长增殖中，组蛋白乙酰化状态的改变情况。方法采用MTT、细胞计数、细胞形态学和Western blot方法，分析DADS对CNE2细胞的增殖抑制作用及与其调控细胞组蛋白乙酰化水平的关系。结果MTT法、细胞计数显示，DADS能明显抑制CNE2细胞增殖，细胞群体倍增时间延长，呈浓度和时间依赖性；HE染色显示，DADS处理CNE2细胞48h后，细胞异型性明显降低，核浆比明显减少；Western blot 分析表明，90μmol/L、140μmol/L、240μmol/L和400μmol/LDADS处理CNE2细胞48h后，组蛋白H3乙酰化的表达较对照组分别增加20%、32%、42%和30%，差异有统计学意义（P<0.05）；组蛋白H4乙酰化程度较对照组差异无统计学意义。结论DADS可抑制CNE2细胞生长增殖，其作用机制可能与组蛋白H3乙酰化水平提高有关。

Effect of Histone Acetylation on Proliferation of CNE2 Cells Inhibited by Diallyl Disulfide

Objective To explore the change conditions of histone acetylation on proliferation of CNE2 cells inhibited by diallyl disulfide (DADS).Methods The growth inhibitions of CNE2 cell line were measured by MTT assay and cell counting.Expression of H3 and H4 was determined by western blot method.Results The results from MTT assay and cell counting showed that DADS significantly inhibited the growth of CNE2 cells.Light microscope examination indicated that the heteromorphism and karyoplasmic ratio decreased.The re...