下调解耦联蛋白2表达抑制Fadu细胞增殖

目的 探讨解耦联蛋白2（UCP2）对下咽癌Fadu细胞的影响,及UCP2对细胞周期以及细胞增殖的影响;同时探讨临床常用化疗药物顺铂（cisplatin）对UCP2基因下调后的Fadu细胞增殖的影响。 方法 流式细胞术/PI染色法检测UCP2基因对Fadu细胞周期的影响;集落形成实验检测UCP2基因下调对Fadu细胞增殖的影响;MTT法和Brdu法检测UCP2能否增强Fadu细胞对顺铂的敏感性以及使用UCP2抑制剂京平尼（genipin）处理细胞后,Fadu细胞的增殖情况。 结果 干扰UCP2表达抑制Fadu细胞周期于G₁/S期;抑制细胞集落形成,但干扰UCP2表达对cisplatin抑制Fadu增殖无协同作用;genipin抑制UCP2的表达后,细胞增殖受到抑制,具有时间/浓度依赖性。 结论 下调UCP2的表达后,Fadu细胞的增殖能力减弱,且抑制细胞周期于G₁/S期,提示UCP2可能在下咽癌Fadu细胞增殖过程中发挥重要的调节作用。

Down-regulated uncoupling protein 2 expression suppresses Fadu cell proliferation

Objective The effect of uncoupling protein 2(UCP2) on the Fadu cells of the hypopharyngeal cancer was investigated in this study. The siRNA technology was used to study the effect of UCP2 on cell cycle and cell proliferation. Meanwhile, cisplatin, a commonly chemotherapeutic drug, was researched on the effect of proliferation in Fadu cells after down-regulated UCP2 gene. Methods Flow cytometry/PI staining was used to observe the effect of UCP2 on Fadu cell cycle. Clone formation assay was used to detect the effect of down-regulated UCP2 on the proliferation of Fadu cells. MTT assay and Brud assay were used to determine whether UCP2 enhanced the sensitivity of Fadu cells to cisplatin and to detect the effect of UCP2 on the proliferation of Fadu. Results The present result showed that down-regulated UCP2 expression inhibited Fadu cell cycle in G₁/S phase and cell colony formation, but had no synergistic effect on inhibition of Fadu proliferation by cisplatin; The expression of UCP2 was significantly inhibited by genipin, and then inhibited cell proliferation in a time-concentration-dependent manner. Conclusion The proliferation of Fadu cells was weakened and the cell cycle was inhibited in G₁/S phase after down-regulated UCP2, suggesting that UCP2 may play an important adjust role in the development in Fadu of hypopharyngeal cancer.