| 艾司西酞普兰对MPTP处理的PC12细胞的保护作用及机制 |
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| 引用本文： | 李娜,张君,刘俊骞,张赛,刘惠苗,王文婷,张忠霞,仇福成,顾平.艾司西酞普兰对MPTP处理的PC12细胞的保护作用及机制[J].解剖学杂志,2019,42(1):31-35. |
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| 作者姓名： | 李娜 张君 刘俊骞 张赛 刘惠苗 王文婷 张忠霞 仇福成 顾平 |
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| 作者单位： | 河北医科大学第一医院神经内科,石家庄050031;河北医科大学第一医院脑功能科,石家庄050031;河北医科大学第一医院细胞治疗实验室,石家庄,050031;河北医科大学第一医院脑功能科,石家庄,050031;河北医科大学第一医院神经内科,石家庄,050031;河北医科大学第一医院神经内科,石家庄050031;河北省脑老化与认知神经科学实验室,石家庄050031 |
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| 摘 要： | 目的 :探讨艾司西酞普兰(ESC)对1-甲基-4-苯基-1,2,3,6-四氢吡啶(MPTP)处理的PC12细胞的保护作用及其机制。方法 :用不同浓度ESC或/和不同浓度MPTP处理PC12细胞,再用3-甲基腺嘌呤(3-MA)抑制;通过MTS检测细胞存活率,Hoechst 33258染色观察细胞凋亡,免疫印迹检测自噬标记物LC3B-Ⅱ、LC3B-Ⅰ和凋亡蛋白Bcl-2、Bax的表达。结果 :经筛选得出,10μmol/L ESC预处理PC12细胞1 h,再加入1 000μmol/L MPTP处理24 h,细胞存活率显著提高;Hoechst 33258染色显示细胞核固缩明显减少;ESC+MPTP组LC3B-Ⅱ/Ⅰ、Bcl-2/Bax表达水平较MPTP组显著上升,但ESC+MPTP+3MA组LC3B-Ⅱ/Ⅰ、Bcl-2/Bax表达水平均显著下降。结论 :ESC对MPTP处理的PC12细胞有一定的神经保护作用,可能通过PI3K/Akt途径激发自噬作用,提高了Bcl-2的表达,抑制MPTP引发的细胞凋亡,从而发挥对其保护作用。
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| 关 键 词： | 帕金森病 艾司西酞普兰 自噬 PC12细胞 1-甲基-4-苯基-1 2 3 6-四氢吡啶 3-甲基腺嘌呤 |
Protective effect and mechanism of estalopram on PC12 cells treated with MPTP |
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| Li Na,Zhang Jun,Liu Junqian,Zhang Sai,Liu Huimiao,Wang Wenting,Zhang Zhongxia,Qiu Fucheng,Gu Ping.Protective effect and mechanism of estalopram on PC12 cells treated with MPTP[J].Chinese Journal of Anatomy,2019,42(1):31-35. |
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| Authors: | Li Na Zhang Jun Liu Junqian Zhang Sai Liu Huimiao Wang Wenting Zhang Zhongxia Qiu Fucheng Gu Ping |
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| Institution: | (Department of Neurology,First Hospitalof Hebei Medical University,Shijiazhuang 050031,China;Cell Therapy Laboratory,First Hospitalof Hebei Medical University,Shijiazhuang 050031,China;Department of Brain Function,First Hospitalof Hebei Medical University,Shijiazhuang 050031,China;Brain Aging and Cognitive Neuroscience Laboratory of Hebei Province,First Hospitalof Hebei Medical University,Shijiazhuang 050031,China) |
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| Abstract: | Objective:To investigate the protective effect and mechanism of estalopram(ESC)on PC12 cells treated with 1-methyl-4-phenyl-6-tetrahydropyridine(MPTP).Methods:PC12 cells were treated with different concentrations of ESC or/and MPTP and then inhibited by 3-methyladenine(3-MA).The cell viability rate was detected by MTS.Cell apoptosis was detected by Hoechst 33258 staining.The expression of autophagy marker LC3B-Ⅱ,LC3B-Ⅰand apoptotic protein Bcl-2,Bax were detected by Western blotting.Results:PC12 cells were pretreated with 10μmol/L ESC for 1 h,and then treated with 1 000μmol/L MPTP for 24 h.The cell viability rate was significantly increased.Hoechst 33258 staining showed that nuclear pyknosis was significantly decreased.The expression of LC3B-Ⅱ/Ⅰand Bcl-2/Bax in ESC+MPTP group was significantly higher than that in MPTP group,but the expression of LC3B-Ⅱ/Ⅰand Bcl-2/Bax in ESC+MPTP+3MA group was significantly lower than that in MPTP group.Conclusion:ESC has a neuroprotective effect on PC12 cells treated with MPTP,which may stimulate autophagy through PI3K/Akt pathway,increase the expression of Bcl-2 and inhibit the apoptosis induced by MPTP. |
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| Keywords: | Parkinson's disease escitalopram autophagy PC12 cell 1-methyl-4-phenyl-1-trimethyl-6-tetrahydropyridine 3-methyladenine |
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