| 骨髓基质细胞对白血病细胞株的保护作用 |
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| 引用本文： | 李忠俊,陈幸华,滕本秀,张琨琨. 骨髓基质细胞对白血病细胞株的保护作用[J]. 中国输血杂志, 2006, 19(2): 104-106 |
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| 作者姓名： | 李忠俊 陈幸华 滕本秀 张琨琨 |
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| 作者单位： | 第三军医大学附属新桥医院,重庆,400037 |
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| 基金项目： | 国家自然科学基金资助项目(No:30070327、No:39770335)，第三军医大学创新基金资助项目(2004D110) |
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| 摘 要： | 目的研究原代正常骨髓基质细胞与白血病细胞耐药、抗凋亡的关系。方法应用Percoll分离骨髓基质细胞14份,设实验组体外与白血病细胞共培养组,模拟骨髓微环境功能,另设单独悬浮培养组;同时没自发凋亡组做对照。AnnexinV/PI双标法检测3组白血病细胞凋亡率。结果共培养后骨髓基质细胞抑制药物诱导的白血病细胞凋亡率较对照组显著降低(P<0.005)。结论骨髓基质细胞对白血病细胞有保护作用。
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| 关 键 词： | 微环境,骨髓 白血病细胞凋亡 白血病,残留 |
| 文章编号： | 1004-549X（2006）02-0104-03 |
| 收稿时间： | 2005-11-04 |
| 修稿时间： | 2006-04-04 |
In vitro protection of Jurkat cells from daunorubicin induced apoptosis by normal bone marrow stromal cells |
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| LI Zhongjun , CHEN Xinghua , TENG Benxiu ,et al.. In vitro protection of Jurkat cells from daunorubicin induced apoptosis by normal bone marrow stromal cells[J]. Chinese Journal of Blood Transfusion, 2006, 19(2): 104-106 |
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| Authors: | LI Zhongjun CHEN Xinghua TENG Benxiu et al. |
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| Affiliation: | Xinqiao Hospital, the affiliated hospital of the third Military Medical University, Chongqing 400037 ,China |
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| Abstract: | Objective To study the influence of bone marrow microenvironment on leukemic cells during chemotherapy and the influence on leukemic cells' drug-resistance and anti-apoptosis. Methods Normal bone marrow stromal cells were isolated using Percoll, and then were cocultured with human acute lymphocyte leukemic cell line Jurkat cells in vitro. Apoptosis percentage of Jurkat cells labelled with Annexin V/PI were detected by FCM. Results Apoptosis percentage of Jurkat cells cocultured with bone marrow stromal cells for 24 hours was lower than those maintained in suspension (P<0.005). Conclusion Bone marrow stromal cells inhibit leukemic cells apoptosis induced by daunorubicin and protect leukemic cells against chemotherapy. |
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| Keywords: | Bone marrow microenvironment Apoptosis Minimal residual leukemia |
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