二羟丙腺苷加高压氧对小鼠脑炎病毒感染的抑制作用

目的 :研究S型与RS型二羟丙腺苷 (DHPA)与高压氧联用对人工感染小白鼠体内日本乙型脑炎 (乙脑 )病毒的抑制作用。方法 :整个实验用两株不同乙脑病毒分两次进行。每次一株 ,乙脑病毒接种小白鼠后将其分组 ,接种后 4～ 6h开始治疗 ,连续 5d ,于开始治疗后第二周末结束实验 ,计算每组小鼠死亡与存活数。结果 :对照组与 (S) DHPA/高压氧联合治疗组及 (RS) DHPA/高压氧联合治疗组效果差异有高度显著性 (P分别 <0 .0 2 5及 <0 .0 1) ,对照组与其他组治疗效果差异无显著性 (P均 >0 .0 5 )。结果 :(S) DHPA或 /及 (RS) DHPA与高压氧联合治疗可抑制人工感染小鼠体内日本乙型脑炎病毒的致病作用。

Inhibitory effect of the combination of DHPAs and hyperbaric oxygen on Japanese B encephalitis virus infection in mice

Objective　To investigate the inhibitory effect of the combination of 2,3-dihydroxypropyl-adenine (DHPAs) and hyperbaric oxygen (HBO) on Japanese B encephalitis virus infection in mice. Methods　Mice infected P3-55 and P3-53 virus were used in the experiments. Virus was inoculated intraperitoneally to Kumming strain mice (8.5～9.5*!g). Mice were divided into groups and treated in the incubation period of the infection. The duration of treatment was 5 days, and the experiments were terminated at the end of 2 weeks after treatment. The living and the dead were then counted finally. Results　The intergroup comparison showed marked differrence between the control group and combination of DHPAs and hyperbaric oxygen gruop (P<0.025 and P<0.01), but there was no statistical difference between other gruops and the control group (P>0.05). Conclusion　The combined treatment of (S) DHPA and/or (RS)-DHPA and HBO has inhibitory effect on Japanese encephalitis virus infection in experimental mice.