尿液端粒酶活性在膀胱癌诊断和监测复发中的意义

目的　探讨检测尿液中端粒酶活性在膀胱癌诊断和监测复发中的意义。　方法　采用PCR ELISA法检测 4 6例膀胱癌患者尿液中的端粒酶活性 ,并观察其中 2 0例术后尿液端粒酶活性变化及与复发的关系。　结果　膀胱癌患者术前尿液端粒酶活性 0 .6 0± 0 .5 6 ,明显高于对照组 0 .18± 0 .0 8,P <0 .0 0 1,肿瘤切除后恢复正常 ,肿瘤复发前再次上升 ;端粒酶活性随肿瘤恶性程度的增加逐渐升高 ;术前端粒酶活性与早期复发无关。　结论　尿液端粒酶活性检测在膀胱癌诊断中有参考价值 ,与膀胱癌恶性程度相关 ,可能成为监测肿瘤复发的重要辅助手段。

Urinary telomerase activity in the diagnosis and recurrence screening of bladder carcinoma

Objective To investigate the significance of urinary telomerase activity (TA) in the diagnosis and recurrence screening of bladder carcinoma. Methods TA in urine of 46 cases of superficial bladder carcinoma and 46 normal subjects was estimated by the method of PCR ELISA, and it was monitored continuously in 20 cases after transurethral resection of tumors. Results TA in urine of patients with bladder carcinoma increased significantly as compared with the controls.It dropped to normal range after tumor removal, but rose again with tumor recurrence.The specificity and sensitivity of TA detection were 93.5% and 47.8% respectively. TA was positively related to the grade of tumors whereas there was no association between the preoperative value of TA and early recurrence. Conclusions The examination of TA in urine is helpful for the diagnosis of bladder carcinoma and it is related to the differentiation degree of tumors.It may become an important supplement to cystoscopy in the screening of tumor recurrence.