H37Rv结核标准菌株构建豚鼠膝关节滑膜结核模型的研究

目的探索利用H37Rv结核标准菌株构建豚鼠膝关节滑膜结核病理模型的方法。方法对经过福氏佐剂免疫和致敏的豚鼠,行膝关节腔H37Rv菌株两种菌量(1×107/mL,50μL及100μL)的注射感染,观察豚鼠感染结核菌后膝关节滑膜组织及关节软骨与骨的病理变化,并行膝关节滑膜集菌培养。结果豚鼠膝关节在行两种菌量的H37Rv菌株感染后,局部肿胀均较明显,但动物的全身反应都较轻微。两组动物的膝关节滑膜病理切片均显示有典型的结核结节及其内的干酪样坏死灶形成;切片抗酸染色均检出有抗酸染色阳性的分枝杆菌;两组动物的关节滑膜匀浆培养均有结核分枝杆菌生长。结论通过在经预先免疫与致敏豚鼠的膝关节局部进行适当剂量的H37Rv结核菌株的注射感染,可以构建出与人类滑膜结核病理变化相似的豚鼠膝关节滑膜结核。

Establishment of the Guinea Pig Model of Synovial Tuberculosis of Knee Joint by Mycobacterium Tuberculosis H37Rv Infection

OBJECTIVE: To explore how to establish the guinea pig model of synovial tuberculosis of knee joint by mycobacterium tuberculosis H37Rv infection. METHODS: Two doses (1 x 10(7)/mL, 50 microL and 100 microL) of Mycobacterium strains were injected into the knee joint of the guinea pig that had been immunized and allergized with Freund's complete adjuvant six weeks previously. The pathological changes of the synovial membrane, cartilage and bone after infection of mycobacterium tuberculosis were investigated. At the same time, the synovial membrane tissues and culture for the mycobacterium tuberculosis were collected and examined. RESULTS: After the infection of mycobacterium tuberculosis H37Rv, the knee joint of guinea pig became swollen, but the interference to the whole conditions of the guinea pig was slight in both infection groups. The tissue sections of the synovial membrane showed tubercle and caseous necrosis of the knee joint in both groups. The mycobacterium tuberculosis could be detected by using Ziehl-neelsen stain and the culture from the synovial membrane of the knee joint. CONCLUSION: By injecting suitable dosage of mycobacterium H37Rv strains into the joint of the immunized and allergized guinea pig, we have established the guinea pig model of synovial tuberculosis that is pathologically similar to synovial tuberculosis in humans, so it is fit for use in the diagnostic studies and pathological researches.