| Q3衰老小鼠模型的DNA损伤研究 |
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| 引用本文： | 幸浩洋,胡新珉,等.Q3衰老小鼠模型的DNA损伤研究[J].华西医科大学学报,2001,32(2):229-231. |
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| 作者姓名： | 幸浩洋 胡新珉 |
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| 摘 要： | 目的 探讨自由基致衰老的小鼠模型DNA氧化损伤的情况。方法 通过给2月龄小鼠吸入一定量的O3，建立O3衰老小鼠模型；然后以单细胞凝胶电泳检测O3衰老小鼠模型、自然衰老小鼠和正常青年小鼠（阴性对照 组）的脾淋巴细胞DNA氧化损伤情况。结果 与阴性对照组相比，O3衰老小鼠模型的脾淋巴细胞DNA受到较显著的氧化损伤（P＜0．05）；其损伤程度与自然衰老小鼠相近（P＞0．05）。结论 O3衰老动物模型与自然衰老近似，在衰老的研究和抗衰老药物的药理实验中具有一定应用价值。
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| 关 键 词： | 臭氧 衰老模型 自由基 DNA损伤 单细胞凝胶电泳 |
Study on DNA oxidative damage of O3 aging model in mice] |
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| H Xing,X Hu,H Liu,Y Li,Y Chen.Study on DNA oxidative damage of O3 aging model in mice][J].Journal of West China University of Medical Sciences,2001,32(2):229-231. |
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| Authors: | H Xing X Hu H Liu Y Li Y Chen |
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| Institution: | Department of Physics, School of Basic Medical Sciences, WCUMS, Chengdu 610041, China. |
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| Abstract: | OBJECTIVE: This study was aimed to detect the animals' DNA oxidative damage. METHODS: A free radical mouse aging model was established by putting the mouse in a device for inhaling quantities of ozone (O3). Single cell gel electrophoresis (SCGE) was used for assessing the splenolymphocytes' DNA damage. RESULTS: The appearance rate of comet and the length grade of DNA migration for the young control group (P < 0.05). No significant difference in the degree of DNA damage was observed between the O3 model group and the natural aging group(P > 0.05). CONCLUSION: The DNA damage of O3 animal model is consistent with that of natural aging animal; this animal model DNA damage of O3 animal model is consistent with that of natural aging animal; this animal model and the method of SCGE are useful for future researches on aging and antiaging medicine. |
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