鼻咽癌患者血清和癌组织中乳酸脱氢酶及其同工酶的研究

目的：研究鼻咽癌（ＮＰＣ）患者血清和癌组织中乳酸脱氢酶（ＬＤＨ）及其同工酶的水平。方法；采用浓度梯度聚丙酰胺弟胶电泳技术检测鼻咽癌患者和对照组血清和癌组织中ＬＤＨ及其同工酶浓度。结果：鼻咽癌组与非癌各对照组的血清ＬＤＨ总酶活力比较无显著差异。但癌组织匀浆ＬＤＨ总酶活力明显高地非癌各组鼻咽部炎性组，鼻咽癌组血清ＬＤＨ同工酶谱显示ＬＤＨ３明显升高，与非癌各组比较有显著差异，且与病变严重程度成正相关，鼻

A STUDY ON LDH LEVEL AND ITS ISOENZYMES IN SERA AND NEOPLASTIC TISSUES OF PATIENTS WITH NASOPHARYNGEAL CARCINOMA

Objective:To study LDH level and its isoenzymes in sera and neoplastic tissues of patients with nasopharyngeal carcinoma (NPC) Methods:Polyacrylamide gel electrophoresis (PAGE) technique was used to assay LDH total level and its isoenzymes of sera and nasopharyngeal tissues Results:There was no difference among patients of NPC and other groups in total activity of serum LDH The total LDH activity of neoplastic tissues was significantly higher than that of inflammatory tissues ( P <0 05) The patterns of serum LDH isoenzymes in NPC group were charecterized by a relative increase of LDH 3 There was significant difference between NPC group and healthy control group ( P <0 05) In patients with benign lesion and unrecurrent NPC,the isoenzymes patterns of sera were similar to those of the healthy control group The isoenzyme patterns of neoplastic tissues were increase in LDH 3,LDH 4 and LDH 5 The LDH total activity and its isoenzymes patterns of cancer tissue in untreated and recurreut NPC groups were different from those in unrecurrent NPC and benign lesions of heads and necks Conclusion:The assay of LDH total level and patterns of its isoenzymes of serum and nasopharyngeal tissue will be useful for disgnosis of NPC,investigation of the effect of treatmert and progrosis in patients of NPC