|
|||||
|
|
| 以绿色荧光蛋白为报告基因的酿酒酵母表达载体的构建 | |
| 引用本文： | 王弘,郑文岭,杨连生,于淑娟,赖晃文,杨传红,马文丽. 以绿色荧光蛋白为报告基因的酿酒酵母表达载体的构建[J]. 广东医学, 2002, 23(12): 1239-1240 |
| 作者姓名： | 王弘 郑文岭 杨连生 于淑娟 赖晃文 杨传红 马文丽 |
| 作者单位： | 1. 华南理工大学食品与生物工程学院,广州,510064 2. 广州军区广州总医院医学实验科,广州,510010 3. 第一军医大学分子生物研究所,广州,510515 |
| 基金项目： | 国家自然科学基金资助项目(编号:39880044)，广州市重大科技项目(编号:199-2005-001) |
| 摘 要： | 目的：构建以绿色荧光蛋白（GFP）为报告基因的酿酒酵母表达载体。方法：利用通用载体质粒融合系统（UPS），首先将GFP片断连入donor载体，随后在Cre酶作用下与acceptor载体融合，获得了带有GFP片断的酿酒酵母（saccharomyces cerevisiae）表达载体。结果：成功构建了以GFP为报告基因的酿酒酵母载体，并在酵母中得到表达。结论：GFP是一种理想的报告基因。同时，利用UPS能够高效、简便地进行酵母表达载体的构建。 |
| 关 键 词： | 酿酒酵母 绿色荧光蛋白 酵母表达载体 通用载体质粒融合系统 GFP |
| 修稿时间： | 2002-07-01 |
Construction of saccharomyces cerevisiae expression vector with GFP as report gene |
|
| Wang Hong,Zhen Wenling,Yang Lian-sheng,et al.. Construction of saccharomyces cerevisiae expression vector with GFP as report gene[J]. Guangdong Medical Journal, 2002, 23(12): 1239-1240 | |
| Authors: | Wang Hong Zhen Wenling Yang Lian-sheng et al. |
| Affiliation: | Wang Hong,Zhen Wenling,Yang Lian-sheng,et al. Food and Biological Engineering College,South China University of Technology,Guangzhou 510640 |
| Abstract: | Objective To construct saccharomyces cerevisiae expression vector with GFP as report gene. Methods By using UPS( univector plasmid - fusion system), GFP gene was linked into donor vector. Saccharomyces cerevisiae expression vector was formed after fusion of donor vector and acceptor vector under the catalyzation of Cre enzyme. Results Saccharomyces cerevisiae expression vector with GFP as report gene was constructed and expressed successfully. Conclusion GFP is an ideal report gene, UPS is efficient and converient for construction of expressing vector. |
| Keywords: | GFP gene Saccharomyces cerevisiae expression vector Univector plasmid- fusion system |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |