TUNEL法检测胰腺癌细胞凋亡和Ki-67表达的研究

为探讨胰腺癌细胞Ki 67与细胞凋亡的关系 ,通过 1 ,2 5 (OH) 2 D3诱发胰腺癌细胞株Bxpc 3凋亡 ,采用MTT法检测细胞增生情况 ,TUNEL法检测细胞凋亡指数 ,免疫组化法检测Ki 67阳性表达指数 ,并进行相关性分析。结果 :在 1 0 - 8mol/L 1 ,2 5 (OH) 2 D3诱发Bxpc 3凋亡过程中 ,肿瘤细胞凋亡指数与Ki 67阳性表达指数呈负相关。提示 :对凋亡指数及Ki 67阳性表达指数的综合分析 ,可全面反映细胞凋亡和增生的平衡状态 ,对于判断肿瘤细胞的增生具有参考价值

Study on the Expression of Ki-67 and Apoptosis in the Pancreas Cancer Cell Line Bxpc-3

To test the realtionship between Ki-67 index and apoptosis in the pancreas cancer cell line Bxpc-3, apoptosis was inducted by 1,25(OH) 2D 3 in the pancreas cancer cell line. We compared the cell number by MTT, determined quantitatively apoptosis cells by TUNEL, detected the expression of Ki-67 index by immunohistochemical. Results suggested that the expression of the Ki-67 index was decreased during apoptosis induced by 10 -8 mol/L 1,25(OH) 2D 3 in Bxpc-3 cell line. There was negative correlation between the apoptosis and the expression of Ki-67 index. Comprehensive analysis of the apoptosis and Ki-67 index reflects the balance of proliferation and apoptosis.