Incorporation of PLGA nanoparticles into porous chitosan-gelatin scaffolds: influence on the physical properties and cell behavior |
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Authors: | Nandagiri Vijay Kumar Gentile Piergiorgio Chiono Valeria Tonda-Turo Chiara Matsiko Amos Ramtoola Zeibun Montevecchi Franco Maria Ciardelli Gianluca |
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Affiliation: | aDepartment of Mechanics, Politecnico di Torino, Corso Duca degli Abruzzi 24, 10129 Turin, Italy;bSchool of Pharmacy, Royal College of Surgeons in Ireland, 123, St. Stephen Green, Dublin 2, Ireland;cDepartment of Anatomy, Royal College of Surgeons in Ireland, 123, St. Stephen Green, Dublin 2, Ireland |
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Abstract: | Bone regeneration can be accelerated by localized delivery of appropriate growth factors/biomolecules. Localized delivery can be achieved by a 2-level system: (i) incorporation of biomolecules within biodegradable particulate carriers (nanoparticles), and (ii) inclusion of such particulate carriers (nanoparticles) into suitable porous scaffolds. In this study, freeze-dried porous chitosan–gelatin scaffolds (CH–G: 1:2 ratio by weight) were embedded with various amounts of poly(lactide-co-glycolide) (PLGA) nanoparticles, precisely 16.6%, 33.3% and 66.6% (respect to CH–G weight). Scaffolds loaded with PLGA nanoparticles were subjected to physico-mechanical and biological characterizations including morphological analysis, swelling and dissolution tests, mechanical compression tests and cell viability tests. Results showed that incorporation of PLGA nanoparticles into porous crosslinked CH–G scaffolds: (i) changed the micro-architecture of the scaffolds in terms of mean pore diameter and pore size distribution, (ii) reduced the dissolution degree of the scaffolds, and (iii) increased the compressive modulus. On the other hand, the water uptake behavior of CH–G scaffolds containing PLGA nanoparticles significantly decreased. The incorporation of PLGA nanoparticles did not affect the biocompatibility of CH–G scaffolds. |
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Keywords: | Chitosan Gelatin Genipin Poly(lactide-co-glycolide) Porous scaffolds Nanoparticles |
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