Anti-inflammatory activity of the constituents of the roots of <Emphasis Type="Italic">Aralia continentalis</Emphasis> |
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Authors: | Hyun Lim Hyun Ah Jung Jae Sue Choi Yeong Shik Kim Sam Sik Kang Hyun Pyo Kim |
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Institution: | 1.College of Pharmacy,Kangwon National University,Chunchon,Korea;2.Division of Food Science and Biotechnology,Pukyong National University,Busan,Korea;3.Natural Products Research Institute and College of Pharmacy,Seoul National University,Seoul,Korea |
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Abstract: | To assess the anti-inflammatory activity of the constituents of the roots of Aralia continentalis, ent-pimara-8(14),15-diene-19-oic acid (continentalic acid, pimaradienoic acid, compound I), 7β-hydroxy-ent-pimara-8(14),15-diene-19-oic acid (compound II), 7-oxo-ent-pimara-8(14),15-diene-19-oic acid (compound III), 15α,16α-epoxy-17-hydroxy-ent-kauran-19-oic acid (compound IV) and ent-kaura-16-en-19-oic acid (kaurenoic acid, compound V), their inhibitory effects against cyclooxygenase-2 (COX-2)-catalyzed
PGE2 and inducible nitric oxide synthase (iNOS)-catalyzed NO production by lipopolysaccharide-treated RAW 264.7 cells were examined.
Among the compounds tested, compound III and V moderately inhibited NO production. In addition, compound III weakly inhibited
PGE2 production, while treatment with compounds II and IV at concentrations of up to 100 μM had no significant effects. Conversely,
compound I only weakly inhibited PGE2 and NO production. To elucidate the mechanism by which these changes occurred, the iNOS down-regulating capacity of compound
III was investigated. Western blot analysis and an electrophoretic mobility shift assay demonstrated that compound III weakly
inhibited COX-2 and iNOS expression at 50–100 μM, and inhibited NF-κB activation. When in vivo anti-inflammatory activities of compounds I, III and V were examined, intraperitoneal injection of 4–100 mg/kg of compound
I and V significantly inhibited carrageenaninduced paw edema in mice, whereas compound III did not. Taken together, the results
of this study suggest that some constituents of A. continentalis, especially compounds I, III and V, exert significant anti-inflammatory activity, which suggests that these constituents
contribute, at least in part, to the anti-inflammatory action of the roots of A. continentalis. |
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