肾细胞癌FHIT基因蛋白编码外显子突变的研究

目的：检测肾细胞癌（ＲＣＣ）组织ＦＨＩＴ基因蛋白编码外显子异常情况，了解ＲＣＣ中ＦＨＩＴ基因异常特征，探讨ＦＨＩＴ基因与ＲＣＣ发生的关系。方法：收集ＲＣＣ及相应癌周肾组织标本２２份和１６份，提取组织ＤＮＡ。ＤＩＧ标记ＦＨＩＴ全长ｃＤＮＡ探针。ＰＣＲ扩增ＦＨＩＴ基因Ｅ５￣Ｅ９，产物行Ｓｏｕｔｈｅｒｎ ｂｌｏｔ杂交。结果：１５份（６８．２％）癌组织标本、６份（３７．５％）癌周肾组织标本存在ＦＨＩＴ基因

Alterations of the human FHIT gene in renal cell carcinoma

Purpose:To detect aberrant of FHIT gene(fragile histidine traid) exons in tissues of RCCs(renal cell carcinoma), surrounding RCCs, and normal kidney, in order to understand the feature of FHIT gene exones alteration in RCC, to inquire into the relationship between FHIT gene and development of RCC.Methods:A total of 22 RCCs and 14 surrounding tissues were obtained from surgical specimens. PCR (polymerase chain reaction) amplications of individual FHIT exons were carried out. PCR products were hybridized with DIG-labeled cDNA probes.Results:Sixty eight percent of RCCs (15/22) and 37.5% (6/16) of surrounding cancer tissues have deletion or aberrant of FHIT exons for protein, and abnormal of FHIT E5 and E8 are much more common than other exons for protein. These alterations usually involve more than one locus of FHIT exon.Conclusion:Lesions at the FHIT DNA level may be the early event in the development of RCC.